Two genetically separable steps in the differentiation of thymic epithelium
Two genetically separable steps in the differentiation of thymic epithelium
复制标题
DOI：
10.1126/science.272.5263.886
复制
发表时间：
1996-05-10
期刊：
影响因子：
56.9
通讯作者：
Boehm, T
中科院分区：
文献类型：
--
作者：
Nehls, M;Kyewski, B;Boehm, T
胸腺的发育最初取决于上皮-间质，随后取决于淋巴-间质的相互相互作用。控制胸腺微环境发育和分化的遗传步骤尚不清楚。通过对再现无胸腺裸鼠表型的whn基因进行靶向破坏，WHN转录因子被证明是裸基因座的产物。在淋巴细胞祖细胞进入之前胸腺上皮原基的形成不需要WHN的活性。然而，原始前体细胞随后分化为出生后胸腺的被膜下、皮质和髓质上皮细胞确实取决于whn基因的活性。这些结果定义了胸腺上皮分化过程中第一个遗传上可分离的步骤。
The development oi the thymus depends initially on epithelial-mesenchymal and subsequently on reciprocal lympho-stromal interactions. The genetic steps governing development and differentiation of the thymic microenvironment are unknown. With the use of a targeted disruption of the whn gene, which recapitulates the phenotype of the athymic nude mouse, the WHN transcription factor was shown to be the product of the nude locus. Formation of the thymic epithelial primordium before the entry of lymphocyte progenitors did not require the activity of WHN. However, subsequent differentiation of primitive precursor cells into subcapsular, cortical, and medullary epithelial cells of the postnatal thymus did depend on activity of the whn gene. These results define the first geneticallyd separable steps during thymic epithelial differentiation.