A prespore gene, Dd31, expressed during culmination of Dictyostelium discoideum.
A prespore gene, Dd31, expressed during culmination of Dictyostelium discoideum.
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前孢子基因 Dd31 在盘基网柄菌达到顶峰时表达。
DOI：
10.1016/0012-1606(91)90421-x
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发表时间：
1991
影响因子：
2.7
通讯作者：
Loomis,WF
中科院分区：
文献类型：
--
作者：
Richardson,DL;Hong,CB;Loomis,WF
在Dictyostelium子实体的顶生过程中，前孢子和前柄细胞经历末端分化，形成孢子和细胞柄。通过随机克隆分离基因组片段，该基因组片段与顶点期间存在的1.4 kb mRNA杂交。细胞类型的分离结果表明，mRNA存在于孢子前细胞和孢子，但不存在于前柄或柄细胞。基因组作图后，克隆了围绕原始1-kb片段的另外3 kb DNA。对该基因进行测序，并根据预测的蛋白产物的大小（以千道尔顿计）命名为Dd 31。Dd 31 mRNA的积累发生在孢子形成之前。添加20 mM 8-Br-cAMP到从墨西哥帽阶段高潮分离的细胞中诱导孢子形成和Dd 31 mRNA的积累，而20 mMcAMP没有。Dd 31 mRNA不积累在同源异型的木霉柄中，其中前孢子细胞转化为柄细胞而不是孢子。Dd 31的表征将已知的分子分化的时间依赖性序列扩展到孢子形成。
During culmination ofDictyosteliumfruiting bodies, prespore and prestalk cells undergo terminal differentiation to form spores and a cellular stalk. A genomic fragment was isolated by random cloning that hybridizes to a 1.4-kb mRNA present during culmination. Cell type separations at culmination showed that the mRNA is present in prespore cells and spores, but not in prestalk or stalk cells. After genomic mapping, an additional 3 kb of DNA surrounding the original 1-kb fragment was cloned. The gene was sequenced and named Dd31 after the size of the predicted protein product in kilodaltons. Accumulation of Dd31 mRNA occurs immediately prior to sporulation. Addition of 20 mM8-Br-cAMP to cells dissociated from Mexican hat stage culminants induced sporulation and the accumulation of Dd31 mRNA, while 20 mMcAMP did not. Dd31 mRNA does not accumulate in the homeotic mutantstalkyin which prespore cells are converted to stalk cells rather than spores. Characterization of Dd31 extends the known temporal dependent sequence of molecular differentiations to sporulation.