Molecular characterization of two-component systems of Helicobactcr pylori
Molecular characterization of two-component systems of Helicobactcr pylori
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DOI：
10.1128/jb.182.8.2068-2076.2000
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发表时间：
2000-04-01
影响因子：
3.2
通讯作者：
Frank, R
中科院分区：
文献类型：
--
作者：
Beier, D;Frank, R
双组分系统经常参与细菌在转录调节水平上对变化的环境条件的适应。在这里，我们报告的两个组件系统的胃病原体幽门螺杆菌推导出菌株26695的基因组序列的成员的表征。我们表明，响应调节器HP 166，HP 1043，和HP 1021具有重要的功能，作为相应的基因的破坏是致命的细菌，不管事实上，BP 100和HP 1021在其接收器域的关键氨基酸的非保守取代。对双组分蛋白的体外磷酸化性质的分析表明，HP 244-HP 703和HP 165-HP 166是同源组氨酸激酶反应调节剂对。此外，我们提供的证据表明，由靠近开放阅读框165/164的3'端的可变长度的多聚（C）区引起的组氨酸激酶HP 165的变异性不会干扰HP 165的递质结构域的激酶活性。
Two-component systems are frequently involved in the adaptation of bacteria to changing environmental conditions at the level of transcriptional regulation. Here we report the characterization of members of the two-component systems of the gastric pathogen Helicobacter pylori deduced from the genome sequence of strain 26695. We demonstrate that the response regulators HP166, HP1043, and HP1021 have essential functions, as disruption of the corresponding genes is lethal for the bacteria, irrespective of the fact that BP100 and HP1021 have nonconserved substitutions in crucial amino acids of their receiver domains. An analysis of the in vitro phosphorylation properties of the two-component proteins demonstrates that HP244-HP703 and HP165-HP166 are cognate histidine kinase-response regulator pairs. Furthermore, we provide evidence that the variability of the histidine kinase HP165 caused by a poly(C) tract of variable length close to the 3' end of open reading frame 165/164 does not interfere with the kinase activity of the transmitter domain of HP165.