Photo-induced inactivation of viruses: Adsorption of methylene blue, thionine, and thiopyronine on Q beta bacteriophage
Photo-induced inactivation of viruses: Adsorption of methylene blue, thionine, and thiopyronine on Q beta bacteriophage
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DOI：
10.1073/pnas.93.15.7446
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发表时间：
1996-07-23
影响因子：
11.1
通讯作者：
Leonard, EF
中科院分区：
文献类型：
--
作者：
Jockusch, S;Lee, D;Leonard, EF
用紫外可见光谱和荧光光谱研究了阳离子有机染料(亚甲蓝、硫氨酸和硫代罗宁)在Qβ噬菌体上的吸附，这些染料对病毒有很强的亲和力，有些染料被用作光敏灭活病毒的敏化剂，在0.1-5mU M的亚甲基蓝浓度范围内，在高染料与病毒的比例(每个病毒粒子大于1000个染料分子)下，这些染料以聚集体的形式结合在病毒上。由于染料的自猝灭，聚集降低了光灭活的效率。在较低的染料与病毒比率(低于每个病毒粒子500个染料分子)下，染料以单体形式与病毒结合。荧光偏振和荧光的时间分辨研究支持基于荧光猝灭的结论。增加离子强度(添加氯化钠)解离结合在病毒上的染料聚集体，并将单体染料释放到主体溶液中。
The adsorption of cationic organic dyes (methylene blue, thionine, and thiopyronine) on Q beta bacteriophage was studied by UV-risible and fluorescence spectroscopy, The dyes have shown a strong affinity to the virus and some have been used as sensitizers for photo-induced inactivation of virus, In the methylene blue concentration range of 0.1-5 mu M and at high ratios of dye to virus (greater than 1000 dye molecules per virion), the dyes bind as aggregates on the virus. Aggregation lowers the efficiency of photoinactivation because of self-quenching of the dye. At lower ratios of dye to virus (lower than 500 dye molecules per virion), the dye binds to the virus as a monomer. Fluorescence polarization and time-resolved studies of the fluorescence support the conclusions based on fluorescence quenching. Increasing the ionic strength (adding NaCl) dissociates bound dye aggregates on the virus and releases monomeric dye into the bulk solution.