Label-free immunodetection with CMOS-compatible semiconducting nanowires
Label-free immunodetection with CMOS-compatible semiconducting nanowires
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DOI：
10.1038/nature05498
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发表时间：
2007-02-01
期刊：
影响因子：
64.8
通讯作者：
Reed, Mark A.
中科院分区：
文献类型：
--
作者：
Stern, Eric;Klemic, James F.;Reed, Mark A.
半导体纳米线具有作为高灵敏度和选择性传感器的潜力，可用于低浓度病原微生物的无标签检测（1-10）。成功的溶液相纳米线传感已经证明离子(3)，小分子(4)，蛋白质（5,6），DNA(7)和病毒(8)；然而，用于这些演示的“自下而上”纳米线（或类似配置的碳纳米管（11））需要混合制造方案（12,13），这导致了严重的集成问题，阻碍了广泛应用。纳米线类器件的替代“自上而下”制造方法（9,10,14-17）由于工艺引起的材料和器件退化而产生令人失望的性能。在这里，我们报告了一种使用互补金属氧化物半导体（CMOS）场效应晶体管兼容技术的方法，因此证明了对低于100飞摩尔浓度的抗体的特异性无标记检测以及对细胞免疫反应的实时监测。这种方法消除了对混合方法的需要，并使这些传感器与信号处理和信息系统的系统级集成成为可能。此外，利用现成的技术实时监测抗体结合和感知细胞免疫反应的能力将促进广泛的诊断应用。
Semiconducting nanowires have the potential to function as highly sensitive and selective sensors for the label-free detection of low concentrations of pathogenic microorganisms(1-10). Successful solution-phase nanowire sensing has been demonstrated for ions(3), small molecules(4), proteins(5,6), DNA(7) and viruses(8); however, 'bottom-up' nanowires ( or similarly configured carbon nanotubes(11)) used for these demonstrations require hybrid fabrication schemes(12,13), which result in severe integration issues that have hindered widespread application. Alternative 'top-down' fabrication methods of nanowire-like devices(9,10,14-17) produce disappointing performance because of process-induced material and device degradation. Here we report an approach that uses complementary metal oxide semiconductor (CMOS) field effect transistor compatible technology and hence demonstrate the specific label-free detection of below 100 femtomolar concentrations of antibodies as well as real-time monitoring of the cellular immune response. This approach eliminates the need for hybrid methods and enables system-scale integration of these sensors with signal processing and information systems. Additionally, the ability to monitor antibody binding and sense the cellular immune response in real time with readily available technology should facilitate widespread diagnostic applications.