A Transcription Factor Code Defines Nine Sensory Interneuron Subtypes in the Mechanosensory Area of the Spinal Cord
A Transcription Factor Code Defines Nine Sensory Interneuron Subtypes in the Mechanosensory Area of the Spinal Cord
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DOI：
10.1371/journal.pone.0077928
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发表时间：
2013-11-04
期刊：
影响因子：
3.7
通讯作者：
Goulding, Martyn
中科院分区：
文献类型：
--
作者：
Del Barrio, Marta Garcia;Bourane, Steeve;Goulding, Martyn
脊髓背侧的中间神经元处理并传递来自感觉触摸、温度和疼痛的皮肤感受器的无害和伤害性躯体感觉信息。这些神经元显示出相对于其传入神经支配的明确的组织。伤害性传入神经支配板层I和II，而皮肤mechanosensory传入神经主要支配位于板层III-IV的感觉中间神经元。在这项研究中，我们概述了一个组合的转录因子代码，定义了9个不同的抑制性和兴奋性的中间神经元群体在层III-IV的出生后的脊髓。这种转录因子编码揭示了构成板层III-IV的神经元中高度的分子多样性，它为系统地分析和操纵这些不同的神经元群体以评估其功能奠定了基础。此外，我们发现许多在出生后早期在脊髓背侧表达的转录因子在成年后继续表达，这就提出了它们在成熟神经元中的功能问题，并为它们在成年动物中的遗传操作打开了大门。
Interneurons in the dorsal spinal cord process and relay innocuous and nociceptive somatosensory information from cutaneous receptors that sense touch, temperature and pain. These neurons display a well-defined organization with respect to their afferent innervation. Nociceptive afferents innervate lamina I and II, while cutaneous mechanosensory afferents primarily innervate sensory interneurons that are located in lamina III-IV. In this study, we outline a combinatorial transcription factor code that defines nine different inhibitory and excitatory interneuron populations in laminae III-IV of the postnatal cord. This transcription factor code reveals a high degree of molecular diversity in the neurons that make up laminae III-IV, and it lays the foundation for systematically analyzing and manipulating these different neuronal populations to assess their function. In addition, we find that many of the transcription factors that are expressed in the dorsal spinal cord at early postnatal times continue to be expressed in the adult, raising questions about their function in mature neurons and opening the door to their genetic manipulation in adult animals.