Determination of the Botanical Origin of Honey by Front-Face Synchronous Fluorescence Spectroscopy
Determination of the Botanical Origin of Honey by Front-Face Synchronous Fluorescence Spectroscopy
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DOI：
10.1366/13-07325
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发表时间：
2014-05-01
影响因子：
3.5
通讯作者：
Bro, Rasmus
中科院分区：
文献类型：
--
作者：
Lenhardt, Lea;Zekovic, Ivana;Bro, Rasmus
正面同步荧光光谱与化学计量学相结合，用于根据蜂蜜样品的植物来源对其进行分类。在 240-500 nm 的激发范围内，同步波长间隔为 30-300 nm，收集了三种单花（椴树、向日葵和金合欢）、多花（草甸混合）和假（假金合欢和椴树）蜂蜜类型（109 个样品）的同步荧光光谱。收集的数据的化学计量分析包括主成分分析和偏最小二乘判别分析。对于仅考虑单花样品的模型和包括单花和多花组的模型，平均交叉验证分类误差分别为 0.2% 和 4.8%。结果表明，不同蜂蜜由于其不同的物理和化学特性，其单次同步荧光光谱存在显着差异，为明确区分蜂蜜类别提供了足够的数据。假蜂蜜样品的光谱与真蜂蜜的光谱存在明显差异，并且根据同步荧光光谱很容易识别这些样品。研究表明，该方法是一种有价值且有前途的蜂蜜认证技术。
Front-face synchronous fluorescence spectroscopy combined with chemometrics is used to classify honey samples according to their botanical origin. Synchronous fluorescence spectra of three monofloral (linden, sunflower, and acacia), polyfloral (meadow mix), and fake (fake acacia and linden) honey types (109 samples) were collected in an excitation range of 240-500 nm for synchronous wavelength intervals of 30-300 nm. Chemometric analysis of the gathered data included principal component analysis and partial least squares discriminant analysis. Mean cross-validated classification errors of 0.2 and 4.8% were found for a model that accounts only for monofloral samples and for a model that includes both the monofloral and polyfloral groups, respectively. The results demonstrate that single synchronous fluorescence spectra of different honeys differ significantly because of their distinct physical and chemical characteristics and provide sufficient data for the clear differentiation among honey groups. The spectra of fake honey samples showed pronounced differences from those of genuine honey, and these samples are easily recognized on the basis of their synchronous fluorescence spectra. The study demonstrated that this method is a valuable and promising technique for honey authentication.