A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death
A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death
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DOI：
10.1039/c0cc02229b
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发表时间：
2011-01-01
影响因子：
4.9
通讯作者：
Ghosh, Indraneel
中科院分区：
文献类型：
--
作者：
Furman, Jennifer L.;Mok, Pui-Wing;Ghosh, Indraneel
设计的传感器包括连接到串联聚（adp -核糖）结合域的分裂萤火虫荧光素酶，可以直接在溶液阶段检测PAR的picogram数量，并监测哺乳动物细胞中聚（adp -核糖）化事件的时间变化。
Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.