Proteomic analysis of serum marker proteins in recipient mice with liver cirrhosis after bone marrow cell transplantation
Proteomic analysis of serum marker proteins in recipient mice with liver cirrhosis after bone marrow cell transplantation
复制标题
DOI：
10.1002/pmic.200500018
复制
发表时间：
2006-04
期刊：
影响因子：
3.4
通讯作者：
Yuichirou Yokoyama;S. Terai;T. Ishikawa;Koji Aoyama;Yohei Urata;Yoshio Marumoto;H. Nishina;K. Nakamura;K. Okita;I. Sakaida
中科院分区：
文献类型：
--
作者：
Yuichirou Yokoyama;S. Terai;T. Ishikawa;Koji Aoyama;Yohei Urata;Yoshio Marumoto;H. Nishina;K. Nakamura;K. Okita;I. Sakaida
我们之前发现，给肝硬化小鼠移植骨髓细胞（BMCs）可改善其肝功能并减轻肝纤维化。在四氯化碳（CCl4）诱导肝损伤的情况下，移植的骨髓细胞迁移至门静脉周围区域，并转分化为可产生白蛋白的肝细胞。因此，在这些条件下，骨髓细胞移植可诱导肝脏再生。检测血清标志物蛋白对于监测肝硬化小鼠骨髓细胞移植后肝功能的恢复情况至关重要。因此，我们首先通过二维电泳（2 - DE）分离了骨髓细胞移植48小时后血清样本中提取的蛋白质，并比较了对照组和骨髓细胞移植组小鼠的蛋白点强度。与对照组相比，骨髓细胞移植组有6个蛋白点强度增加。质谱分析显示，这些蛋白点包括载脂蛋白A1（apoA1）、载脂蛋白C3（apoC3）、维生素D结合蛋白、α - 1 - 抗胰蛋白酶和蛋白酶体α1亚基。随后，我们通过免疫印迹法确认了血清和肝脏样本中apoA1的水平。在该肝硬化小鼠模型中，骨髓细胞移植后早期（48小时和1周）apoA1水平升高。apoA1的早期升高可能有助于预测肝硬化小鼠骨髓细胞移植后的肝脏再生情况。
We previously found that transplantation with bone marrow cells (BMCs) improves liver function and liver fibrosis in cirrhotic mice. In the presence of liver damage induced by carbon tetrachloride (CCl4), transplanted BMC migrated into the peri‐portal region and trans‐differentiated into hepatocytes that produce albumin. Thus under these conditions, BMC transplantation induces liver regeneration. Detecting serum marker proteins is important to monitor the recovery of liver function of cirrhotic mice after BMC transplantation. We therefore initially resolved proteins extracted from serum samples at 48 h after BMC transplantation by 2‐DE and compared spot intensity between control and BMC groups of mice. Six protein spots increased in the BMC group compared with the control group. MS revealed that these spots comprised apolipoprotein A1 (apoA1), apolipoprotein C3 (apoC3), vitamin D‐binding protein, alpha‐1‐antitrypsin and proteasome subunit alpha type 1. We subsequently confirmed the levels of apoA1 in serum and liver samples by immunoblotting. ApoA1 increased at early stage (48 h and 1 wk) after BMC transplantation in this mouse model of liver cirrhosis. The early elevation of apoA1 might be useful to predict liver regeneration in cirrhotic mice after BMC transplantation.