Human milk H2O2 content: does it benefit preterm infants?
Human milk H2O2 content: does it benefit preterm infants?
复制标题
DOI：
10.1038/pr.2017.303
复制
发表时间：
2018-03-01
影响因子：
3.6
通讯作者：
Belik, Jaques
中科院分区：
文献类型：
--
作者：
Cieslak, Monika;Ferreira, Cristina H. F.;Belik, Jaques
背景：人乳含有高含量的抗菌化合物过氧化氢（H2O2）。与健康的足月婴儿不同，早产儿是用以前表达和储存的母乳喂养的。这些做法可能有利于H2O2分解，从而限制了其对早产儿的潜在益处。本研究的目的是评估母乳中H2O2产生和降解的因素。方法：对人乳、大鼠乳及大鼠乳腺组织进行评价。研究了牛奶中催产素和黄嘌呤氧化酶对H2O2生成的影响、H2O2的ph依赖性以及过氧化氢酶和乳过氧化物酶对H2O2的降解作用。结果：乳腺组织黄嘌呤氧化酶负责H2O2的产生，其乳含量依赖于催产素刺激。人乳H2O2含量的稳定性与ph值有关，在酸性范围内稳定性最强。当母乳在室温下保存超过10分钟时，H2O2就会完全降解，这一过程被乳过氧化物酶和过氧化氢酶抑制。结论：新鲜母乳H2O2含量不稳定，常温下降解快。进一步调查母乳处理技术，以保持其H2O2含量，当灌喂给早产儿是必要的。
BACKGROUND: Human milk has a high content of the antimicrobial compound hydrogen peroxide (H2O2). As opposed to healthy full-term infants, preterm neonates are fed previously expressed and stored maternal milk. These practices may favor H2O2 decomposition, thus limiting its potential benefit to preterm infants. The goal of this study was to evaluate the factors responsible for H2O2 generation and degradation in breastmilk.METHODS: Human donors' and rats' milk, along with rat mammary tissue were evaluated. The role of oxytocin and xanthine oxidase on H2O2 generation, its pH-dependent stability, as well as its degradation via lactoperoxidase and catalase was measured in milk.RESULTS: Breast tissue xanthine oxidase is responsible for the H2O2 generation and its milk content is dependent on oxytocin stimulation. Stability of the human milk H2O2 content is pH-dependent and greatest in the acidic range. Complete H2O2 degradation occurs when human milk is maintained, longer than 10 min, at room temperature and this process is suppressed by lactoperoxidase and catalase inhibition.CONCLUSION: Fresh breastmilk H2O2 content is labile and quickly degrades at room temperature. Further investigation on breastmilk handling techniques to preserve its H2O2 content, when gavage-fed to preterm infants is warranted.