Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications.
Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications.
复制标题
DOI：
10.3389/fmolb.2021.653148
复制
发表时间：
2021
影响因子：
5
通讯作者：
Schlundt A
中科院分区：
文献类型：
--
作者：
Altincekic N;Korn SM;Qureshi NS;Dujardin M;Ninot-Pedrosa M;Abele R;Abi Saad MJ;Alfano C;Almeida FCL;Alshamleh I;de Amorim GC;Anderson TK;Anobom CD;Anorma C;Bains JK;Bax A;Blackledge M;Blechar J;Böckmann A;Brigandat L;Bula A;Bütikofer M;Camacho-Zarco AR;Carlomagno T;Caruso IP;Ceylan B;Chaikuad A;Chu F;Cole L;Crosby MG;de Jesus V;Dhamotharan K;Felli IC;Ferner J;Fleischmann Y;Fogeron ML;Fourkiotis NK;Fuks C;Fürtig B;Gallo A;Gande SL;Gerez JA;Ghosh D;Gomes-Neto F;Gorbatyuk O;Guseva S;Hacker C;Häfner S;Hao B;Hargittay B;Henzler-Wildman K;Hoch JC;Hohmann KF;Hutchison MT;Jaudzems K;Jović K;Kaderli J;Kalniņš G;Kaņepe I;Kirchdoerfer RN;Kirkpatrick J;Knapp S;Krishnathas R;Kutz F;Zur Lage S;Lambertz R;Lang A;Laurents D;Lecoq L;Linhard V;Löhr F;Malki A;Bessa LM;Martin RW;Matzel T;Maurin D;McNutt SW;Mebus-Antunes NC;Meier BH;Meiser N;Mompeán M;Monaca E;Montserret R;Mariño Perez L;Moser C;Muhle-Goll C;Neves-Martins TC;Ni X;Norton-Baker B;Pierattelli R;Pontoriero L;Pustovalova Y;Ohlenschläger O;Orts J;Da Poian AT;Pyper DJ;Richter C;Riek R;Rienstra CM;Robertson A;Pinheiro AS;Sabbatella R;Salvi N;Saxena K;Schulte L;Schiavina M;Schwalbe H;Silber M;Almeida MDS;Sprague-Piercy MA;Spyroulias GA;Sreeramulu S;Tants JN;Tārs K;Torres F;Töws S;Treviño MÁ;Trucks S;Tsika AC;Varga K;Wang Y;Weber ME;Weigand JE;Wiedemann C;Wirmer-Bartoschek J;Wirtz Martin MA;Zehnder J;Hengesbach M;Schlundt A
由β冠状病毒SARS-CoV-2引起的高度传染性疾病COVID-19对人类构成严重威胁，需要将科学努力和标准重新定向到有组织的研究项目。国际COVID 19-NMR联盟寻求通过收集全球科学专业知识来提供这种新方法。特别是，获得病毒蛋白和RNA将为详细了解SARS-CoV-2分子组分铺平道路。COVID 19-NMR的研究和通过该联盟提供的资源被充分披露，以加速获取和利用。核磁共振研究的病毒分子组分被指定为进一步的工作，包括大分子相互作用的研究和高通量药物筛选提供必要的基础。在这里，我们提出了一个全面的SARS-CoV-2蛋白制备方法的基础上，该财团的集体努力的广泛目录。我们提供了大规模生产超过80%的SARS-CoV-2蛋白或其重要部分的方案。几种蛋白质是在一个以上的实验室中生产的，这证明了全世界NMR组之间的高度互操作性。对于大多数蛋白质，我们可以生产HSQC级的同位素标记样品。连同在covid19-nmr.com上公开提供的几个NMR化学位移分配，我们在这里为生产同位素标记形式的SARS-CoV-2蛋白提供了非常有价值的资源。
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.
登录
查看更多内容
DOI：
10.1074/jbc.m115.662130
发表时间：
2015-10-16
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chen Y;Savinov SN;Mielech AM;Cao T;Baker SC;Mesecar AD
通讯作者：
Mesecar AD
影响因子：
13.2
作者：
Chan, Jasper Fuk-Woo;Kok, Kin-Hang;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
5.3
作者：
Davies, Jonathan P.;Almasy, Katherine M.;Plate, Lars
通讯作者：
Plate, Lars
影响因子：
1.6
作者：
Bogomolovas, Julius;Simon, Bernd;Stier, Gunter
通讯作者：
Stier, Gunter
影响因子：
56.9
作者：
Anand, K;Ziebuhr, J;Hilgenfeld, R
通讯作者：
Hilgenfeld, R