Penaeicola halotolerans gen. nov., sp. nov., a novel bacterium of the family Cyclobacteriaceae isolated from a shrimp pond
Penaeicola halotolerans gen. nov., sp. nov., a novel bacterium of the family Cyclobacteriaceae isolated from a shrimp pond
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DOI：
10.1099/ijsem.0.005047
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发表时间：
2021-01-01
影响因子：
2.8
通讯作者：
Wang, Hui
中科院分区：
文献类型：
--
作者：
Ali, Sardar;Xie, Jianmin;Wang, Hui
从汕头、中国等地的虾池中分离到一株革兰氏阴性、非游动、杆状需氧细菌，命名为LMIT005(T)。用多相方法对新分离株进行了分类鉴定。根据16S rRNA基因序列分析，菌株LMIT005(T)属于细胞吞噬目的环状细菌科，是一个独立的谱系。菌株LMIT005(T)与该家族中最接近的模式菌株Yomjeoni海藻KCTC 12309(T)的16SRRNA基因序列同源性为91.3%。菌株LMIT005(T)在25℃、pH 7和2.0%(w/v)的氯化钠存在下生长最好。DNA G+C含量(来自基因组序列)为40.5mol%。与参考菌株A.yeomjeoni KCTC 12309(T)相比，LMIT005T的核苷酸同源性为70%。LMIT005T中唯一的呼吸对苯二酚为菜籽酮(MK 7)，主要脂肪酸组成为特征3(C-16：1 omega 6c/C-16：1 omega 7c)。LMIT005T菌株的极性脂主要由磷脂酰乙醇胺、磷脂酰胆碱、两种未知氨基脂、两种未知脂、一种未知糖脂和一种未知磷脂组成。LMIT005(T)株基因组草图全长3089 781个核苷酸(3.09 Mb)，共2773个基因。在LMIT005(T)菌株基因组中发现了与抗生素耐药相关的基因(blal、mexA和mexB)，提示该菌株可能对β-内酰胺类抗生素具有耐药性。药敏试验进一步证实了这一点。由于LMIT005(T)菌株在基因组、形态和生理方面与以前描述的模式菌株有明显的差异，因此它被认为是环菌科的一个新属的代表，命名为Penaeicola halotolans gen。Nov.，sp.11月模式菌株为LMIT005(T)(=KCTC 82616(T)=CICC 25047(T))。
A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterium (designated as LMIT005(T)) was isolated from shrimp ponds in Shantou, China. The new isolate was characterized taxonomically using a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain LMIT005(T) was found to be affiliated with the family Cyclobacteriaceae of the order Cytophagales while appearing as a distinct lineage. The 16S rRNA gene sequence similarity between strain LMIT005(T) and Algoriphagus yeomjeoni KCTC 12309(T), the closest type strain in the family, was 91.3 %. Strain LMIT005(T) grew optimally at 25 degrees C, pH 7 and in the presence of 2.0 % (w/v) NaCl. The DNA G+C content (data from genome sequence) was 40.5 mol%. Compared with reference strain A. yeomjeoni KCTC 12309(T), the average nucleotide identity (ANI) of LMIT005T was 70 %. The sole respiratory quinone of LMIT005T was menaquinone (MK 7), and the major fatty acids were summed feature 3 (C-16:1 omega 6c / C-16:1 omega 7c). The polar lipids of strain LMIT005T were mainly composed of phosphatidylethanolamine, phosphatidylcholine, two unidentified amino lipids, two unidentified lipids, one unidentified glycolipid and one unidentified phospholipid. The draft genome of strain LMIT005(T) comprised 3 089 781 bp (3.09 Mb) nucleotides and 2773 genes. Antimicrobial resistant-related genes (blal, mexA, and mexb) were annotated in the genome of strain LMIT005(T), which indicated that it might be able to resist beta-lactam antibiotics. This was further verified by antimicrobial resistant test. Given its distinct genomic, morphological, and physiological differences from previously described type strains, strain LMIT005(T) is proposed as a representative of a novel genus of the family Cyclobacteriaceae, with the name Penaeicola halotolerans gen. nov., sp. nov. The type strain is LMIT005(T) (=KCTC 82616(T)=CICC 25047(T)).