SENSITIVITY AND DETECTION EFFICIENCY OF THE PEROXIDASE ANTIPEROXIDASE (PAP), AVIDIN BIOTIN PEROXIDASE COMPLEX (ABC), AND PEROXIDASE-LABELED AVIDIN BIOTIN (LAB) METHODS
SENSITIVITY AND DETECTION EFFICIENCY OF THE PEROXIDASE ANTIPEROXIDASE (PAP), AVIDIN BIOTIN PEROXIDASE COMPLEX (ABC), AND PEROXIDASE-LABELED AVIDIN BIOTIN (LAB) METHODS
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DOI：
10.1093/ajcp/92.1.62
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发表时间：
1989-07-01
影响因子：
3.5
通讯作者：
GABORC, D
中科院分区：
文献类型：
--
作者：
ELIAS, JM;MARGIOTTA, M;GABORC, D
作者研究了目前在诊断免疫组织化学中应用最广泛的三种过氧化物酶方法的灵敏度和检测效率：过氧化物酶-抗过氧化物酶（PAP）、抗生物素蛋白-生物素复合物（ABC）和标记抗生物素蛋白-生物素（LAB）方法。通过测定应用于福尔马林固定、石蜡包埋的人胰腺的多克隆抗胰高血糖素抗体的最高有用稀释度来评价灵敏度。通过阳性（三个或更多个阳性细胞）胰岛总数的列表评价检测效率。直接比较，LAB方法在灵敏度和检测效率方面均优于PAP和ABC方法，两者基本相等。PAP法的灵敏度和检测效率均高于ABC法，PAP法与LAB法的灵敏度相当，检测效率高于LAB法。作者得出结论，只有在沿着考虑灵敏度和效率以及任何单一方法的独特要求时，免疫组织学方法的比较才有意义。
The authors have examined the sensitivity and detection efficiency of the three peroxidase methods that currently have the widest application in diagnostic immunohistochemistry: the peroxidase-antiperoxidase (PAP), the avidin-biotin complex (ABC), and the labeled avidin-biotin (LAB) methods. Sensitivity was evaluated by determining the highest useful dilution of polyclonal antiglucagon antibodies applied to formalin-fixed, paraffin embedded human pancreas. Detection efficiency was evaluated by tabulation of the total number of positive (three or more positive cells) islets. On direct comparison, the LAB method exceeded the PAP and ABC methods in both sensitivity and detection efficiency, which were essentially equal. Titration of linking antiserum of the PAP method boosted its sensitivity and detection efficiency above that of ABC; the PAP had equal sensitivity to the LAB and exceeded it in detection efficiency. The authors conclude that comparisons of immmunohistologic methods are meaningful only if both sensitivity and efficiency are considered along with the unique requirements of any single method.