Interaction of C-protein with myosin.
Interaction of C-protein with myosin.
复制标题
C 蛋白与肌球蛋白的相互作用。
DOI：
10.1093/oxfordjournals.jbchem.a132882
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发表时间：
1980
影响因子：
2.7
通讯作者：
H. Noda
中科院分区：
文献类型：
--
作者：
M. Miyahara;H. Noda
用流动双折射、电子显微镜和超离心等方法研究了C蛋白对肌球蛋白组装反应的影响。肌球蛋白丝通过稀释至较低的离子强度形成。在C蛋白存在下形成直径为70-110 A的较细的细丝。当通过适度缓慢稀释（稀释时间为0.5-2分钟）或逐步稀释进行稀释时，C蛋白有利于形成更长的细丝。当通过甚至更慢的稀释（稀释时间超过2分钟）进行稀释时，C蛋白有利于形成更短的细丝。通过缓慢稀释形成的较长的细丝比通过较快稀释形成的较短的细丝并入更多的C-蛋白。将C蛋白加入肌球蛋白丝溶液中，可使肌球蛋白丝结合成更长的肌球蛋白丝。对于较长的长丝，伸长效应较慢且较强。
The effect of C-protein on the assembly reaction of myosin was studied by flow birefringence, electron microscopy, and ultracentrifugation. Myosin filaments were formed by dilution to a lower ionic strength. Thinner filaments of 70-110 A in diameter were formed in the presence of C-protein. When dilution was effected by moderately slow dilution (dilution time of 0.5-2 min) or by stepwise dilution, C-protein favored the formation of longer filaments. When dilution was effected by even slower dilution (dilution time above 2 min), C-protein favored the formation of shorter filaments. Longer filaments formed by slow dilution incorporated more C-protein than shorter ones formed by faster dilution. Addition of C-protein to a solution of myosin filaments caused association of the filaments into longer filaments. The elongation effect was slower and stronger for longer filaments.