Molecular cloning and functional characterization of rat Δ-6 fatty acid desaturase
Molecular cloning and functional characterization of rat Δ-6 fatty acid desaturase
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DOI：
10.1006/bbrc.1999.0235
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发表时间：
1999-02-24
影响因子：
3.1
通讯作者：
Suzuki, O
中科院分区：
文献类型：
--
作者：
Aki, T;Shimada, Y;Suzuki, O
利用表达序列标签（EST）序列信息，获得了编码脂肪酸去饱和酶特征性氨基酸序列的哺乳动物cDNA片段。这些片段随后用于筛选大鼠肝脏cDNA文库，产生1573 bp的克隆。在酵母中表达含有分离克隆的两个可能的开放阅读框（核苷酸编号97-1431和148-1431）中的任一个的DNA片段导致在外源亚油酸存在下γ-亚麻酸的积累。在该系统中，α-亚麻酸的加入也导致其Delta-6去饱和产物的积累，而二高-γ-亚麻酸未能成为底物。这些结果表明，由大鼠cDNA编码的蛋白质是Delta-6脂肪酸去饱和酶，并且对应于克隆的编码区97-147的前17个氨基酸在酵母中不需要发挥功能。(C)北京：科学出版社.
Mammalian cDNA fragments putatively encoding amino acid sequences characteristic of the fatty acid desaturase were obtained using expressed sequence tag (EST) sequence informations. These fragments were subsequently used to screen a rat liver cDNA library, yielding a 1573-bp clone. Expression of DNA fragment containing either of two possible open reading frames (nucleotide numbers 97-1431 and 148-1431) of the isolated clone in yeast led to the accumulation of gamma-linolenic acid in the presence of exogenous linoleic acid. In this system, the addition of alpha-linolenic acid also resulted in the accumulation of its Delta-6 desaturated product whereas dihomo-gamma-linolenic acid failed to be a substrate. These results indicate that the protein encoded by the rat cDNA is Delta-6 fatty acid desaturase, and the first 17 amino acids corresponding to the coding region 97-147 of the clone are not required to function in yeast. (C) 1999 Academic Press.