Calycosin Stimulates Proliferation of Estrogen Receptor-Positive Human Breast Cancer Cells Through Downregulation of Bax Gene Expression and Upregulation of Bcl-2 Gene Expression at Low Concentrations
Calycosin Stimulates Proliferation of Estrogen Receptor-Positive Human Breast Cancer Cells Through Downregulation of Bax Gene Expression and Upregulation of Bcl-2 Gene Expression at Low Concentrations
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DOI：
10.1177/0148607111413900
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发表时间：
2011-11-01
影响因子：
3.4
通讯作者：
Zhou, Li-ming
中科院分区：
文献类型：
--
作者：
Chen, Jian;Xiong, Wen-bi;Zhou, Li-ming
背景：毛蕊花素是中药黄芪的主要成分之一，被认为是一种典型的植物雌激素。它有雌激素或抗雌激素作用，主要取决于体内雌激素水平。本研究探讨了毛蕊异黄酮对雌激素受体（ER）阳性人乳腺癌（MCF-7）细胞的体外作用及其机制。方法：用不同浓度的毛蕊异黄酮处理er阳性MCF-7细胞。MTT法检测毛蕊异黄酮对er阳性MCF-7细胞增殖的影响。流式细胞术检测各组细胞的凋亡情况。用逆转录聚合酶链反应和免疫组织化学染色分别检测细胞中Bcl-2和Bax的mRNA和蛋白水平。结果：与对照相比，低浓度（2、4、8 mu mol/L）的毛蕊异黄酮能促进er阳性MCF-7细胞的增殖。此外，在这些浓度下，毛蕊异黄酮降低了MCF-7细胞的早期凋亡百分比，下调了Bax的mRNA和蛋白水平，并上调了低浓度下Bcl-2的mRNA和蛋白水平。另一方面，较高浓度（16和32 μ mol/L）的毛蕊异黄酮抑制细胞增殖。结论：在较低浓度下，毛蕊异黄酮具有促进MCF-7细胞增殖的作用，其机制可能与雌激素作用有关。（中华儿科杂志。2011;35:763-769）
Background: Calycosin is one of main components in the herb Radix astragali and is considered a typical phytoestrogen. It has either estrogenic or antiestrogenic effects that mainly depend on estrogen levels in vivo. This study investigated the effects and mechanisms of calycosin on estrogen receptor (ER)-positive human breast cancer (MCF-7) cells in vitro. Methods: ER-positive MCF-7 cells were treated with different concentrations of calycosin. Effects of calycosin on the proliferation of ER-positive MCF-7 cells were determined by the MTT assay. Apoptosis in these treated cells was examined by flow cytometry. The mRNA and protein levels of Bcl-2 and Bax in these treated cells were also determined by reverse-transcription polymerase chain reaction and immunohistochemical staining, respectively. Results: Compared with the vehicle control, calycosin stimulated proliferation of ER-positive MCF-7 cells at low concentrations (2, 4, and 8 mu mol/L). Furthermore, at these concentrations, calycosin decreased the percentage of early apoptosis in MCF-7 cells, downregulated mRNA and protein levels of Bax, and upregulated those of Bcl-2 at low concentrations. On the other hand, calycosin at higher concentrations (16 and 32 mu mol/L) inhibited cell proliferation. Conclusion: At relatively low concentrations, calycosin has stimulatory effects on the proliferation of MCF-7 cells, with the estrogenic effect the mechanism. (JPEN J Parenter Enteral Nutr. 2011;35:763-769)