Utilization of star-shaped polymer architecture in the creation of high-density polymer brush coatings for the prevention of platelet and bacteria adhesion.
Utilization of star-shaped polymer architecture in the creation of high-density polymer brush coatings for the prevention of platelet and bacteria adhesion.
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DOI：
10.1039/c4bm00034j
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发表时间：
2014-09-01
影响因子：
6.6
通讯作者：
Tanihara M
中科院分区：
文献类型：
--
作者：
Totani M;Ando T;Terada K;Terashima T;Kim IY;Ohtsuki C;Xi C;Kuroda K;Tanihara M
我们证明了星形聚合物作为高密度聚合物刷涂层的利用及其抑制血小板和细菌粘附的有效性。采用活性自由基聚合法，在钌催化剂作用下合成了聚甲基丙烯酸羟乙酯（PHEMA）和聚甲基丙烯酸甲酯（PMMA）组成的星星型聚合物。通过将聚合物溶液简单滴铸到聚（对苯二甲酸乙二醇酯）（PET）表面上然后干燥来制备聚合物涂层。在本研究中制备的星星聚合物中，PHEMA星星聚合物（star-PHEMA）和PHEMA/PMMA（mol. 71/29）杂臂星星聚合物（星-H71 M29）涂层显示出对血小板粘附（相对于未涂覆的PET表面为78-88%）和大肠杆菌（94-97%）的最高抑制百分比。在Dulbecco磷酸盐缓冲盐水或表面活性剂溶液中孵育7天后，这些涂层还显示出抗血小板的抗粘附活性。此外，星星聚合物的PMMA组分增加了涂层的耐刮擦性。这些结果表明，星形聚合物结构在PET表面上提供高聚合物链密度以防止血小板和细菌的粘附，以及涂层稳定性和物理耐久性以防止暴露裸露的PET表面。星星聚合物提供了一种简单有效的方法来在生物医学材料上制备抗粘附聚合物涂层，以防止血小板和细菌的粘附。
We demonstrate utilization of star-shaped polymers as high-density polymer brush coatings and their effectiveness to inhibit the adhesion of platelets and bacteria. Star polymers consisting of poly(2-hydroxyethyl methacrylate) (PHEMA) and/or poly(methyl methacrylate) (PMMA), were synthesized using living radical polymerization with a ruthenium catalyst. The polymer coatings were prepared by simple drop casting of the polymer solution onto poly(ethylene terephthalate) (PET) surfaces and then dried. Among the star polymers prepared in this study, the PHEMA star polymer (star-PHEMA) and the PHEMA/PMMA (mol. ratio of 71/29) heteroarm star polymer (star-H71M29) coatings showed the highest percentage of inhibition against platelet adhesion (78–88% relative to noncoated PET surface) and Escherichia coli (94–97%). These coatings also showed anti-adhesion activity against platelets after incubation in Dulbecco's phosphate buffered saline or surfactant solution for 7 days. In addition, the PMMA component of the star polymers increased the scratch resistance of the coating. These results indicate that the star-polymer architecture provides high polymer chain density on PET surfaces to prevent adhesion of platelets and bacteria, as well as coating stability and physical durability to prevent exposure of bare PET surfaces. The star polymers provide a simple and effective approach to preparing anti-adhesion polymer coatings on biomedical materials against the adhesion of platelets and bacteria.