Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea.
Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea.
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钟乳杆菌属
DOI：
10.1099/ijs.0.63315-0
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发表时间：
2005
影响因子：
2.8
通讯作者：
Chang
中科院分区：
文献类型：
--
作者：
Jee;C. Jeon;Sung;Chang
从韩国盐场分离到3株中度嗜盐的产孢菌株，分别命名为BH 030062 T、BH 030049和BH 030080。系统发育分析和比较16 S rRNA基因序列研究表明，分离物代表了一个新的独特的单系谱系内的系统群的经典定义为属芽孢杆菌和最密切相关的属的成员纤细芽孢杆菌（93.7- 95.1%相似性）、微芽孢杆菌属（93.5- 94.8%）、盐芽孢杆菌属（94.8- 95.9%）、丝状芽孢杆菌属（94.4- 94.8%）和慢芽孢杆菌属（93.3- 93.7%）。菌株BH 030062 T为严格好氧菌，杆状，革兰氏阳性，能通过周毛鞭毛运动。它在1- 15%（w/v）NaCl的存在下和15-45 ℃的温度下生长。细胞壁肽聚糖含有A1 γ-内消旋-二氨基庚二酸作为诊断性二氨基酸。主要的细胞脂肪酸是异-C（15：0）、反异-C（15：0）和异-C（16：0）。DNA G+C含量约为41mol%，主要的类异戊二烯醌是MK-7。根据其生理和分子特性，分离物代表一个新属，Pontibacillus gen. nov.，和新种，Pontibacilluschungwhensis sp. nov。模式菌株为BH 030062 T（=KCTC 3890 T =DSM 16287 T）。
Three moderately halophilic, spore-forming strains, designated BH030062T, BH030049 and BH030080, were isolated from a solar saltern in Korea. Phylogenetic analyses and comparative 16S rRNA gene sequence studies revealed that the isolates represent a novel distinct monophyletic lineage within the phyletic group classically defined as the genus Bacillus and are most closely related to members of the genera Gracilibacillus (93.7-95.1 % similarity), Virgibacillus (93.5-94.8 %), Halobacillus (94.8-95.9 %), Filobacillus (94.4-94.8 %) and Lentibacillus (93.3-93.7 %). Strain BH030062T was strictly aerobic, rod-shaped, Gram-positive and motile by means of peritrichous flagella. It grew in the presence of 1-15 % (w/v) NaCl and at temperatures of 15-45 degrees C. The cell wall peptidoglycan contained A1gamma-meso-diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids were iso-C(15 : 0), anteiso-C(15 : 0) and iso-C(16 : 0). DNA G+C content was about 41 mol% and the major isoprenoid quinone was MK-7. On the basis of their physiological and molecular properties, the isolates represent a new genus, Pontibacillus gen. nov., and novel species, Pontibacillus chungwhensis sp. nov. The type strain is BH030062T (=KCTC 3890T=DSM 16287T).