Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model
Intra-arterially infused carbon dioxide-saturated solution for sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model
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DOI：
10.3892/ijo.2017.4056
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发表时间：
2017-08-01
影响因子：
5.2
通讯作者：
Yamaguchi, Masato
中科院分区：
文献类型：
--
作者：
Katayama, Naoto;Sugimoto, Koji;Yamaguchi, Masato
本研究旨在评价动脉内输注二氧化碳（CO2）饱和溶液对兔VX 2肝肿瘤模型中顺铂抗癌作用的增敏作用。将40只携带VX 2肝肿瘤的日本白色家兔随机分为4组，分别经肝固有动脉灌注生理盐水（对照组）、CO2饱和溶液（CO2组）、顺铂溶液（顺铂组）或CO2饱和溶液和顺铂溶液（联合组）。使用对比增强计算机断层扫描计算肿瘤体积（TV）和相对肿瘤体积（RTV），RTV =（第3或7天的TV）/（第0天的TV）x 100。缺氧诱导因子-1 α（HIF-α）和碳酸酐酶IX（CA IX）染色用于评估细胞缺氧。分析裂解的半胱天冬酶-3和裂解的半胱天冬酶-9以评估肿瘤细胞凋亡。对照组第3天和第7天的平均RTV分别为202.6 +/- 23.7和429.2 +/-94.8%; CO2组分别为172.2 +/- 38.1和376.5 +/- 61.1%;顺铂组分别为156.1 +/- 15.1和269.6 +/- 45.2%;联合组分别为118.3 ± 28.1和210.3 ± 55.1%。CO2组的RTV显著低于对照组（第3天; P
The present study aimed to evaluate the efficacy of an intra-arterially infused carbon dioxide (CO2)-saturated solution in sensitizing the anticancer effect of cisplatin in a rabbit VX2 liver tumor model. Forty VX2 liver tumor-bearing Japanese white rabbits were randomly divided into four groups and infused via the proper hepatic artery with a saline solution (control group), CO2 saturated solution (CO2 group), cisplatin solution (cisplatin group), or CO2-saturated solution and cisplatin solution (combined group). The tumor volume (TV) and the relative tumor volume (RTV), RTV = (TV on day 3 or 7)/(TV on day 0) x 100, were calculated using contrast enhanced computed tomography. Hypoxia-inducible factor-1 alpha (HIF-alpha) and carbonic anhydrase IX (CA IX) staining were used to evaluate cellular hypoxia. Cleaved caspase-3 and cleaved caspase-9 were analyzed to assess tumor apoptosis. The mean RTV on days 3 and 7 were 202.6 +/- 23.7 and 429.2 +/- 94.8%, respectively, in the control group; 172.2 +/- 38.1 and 376.5 +/- 61.1% in the CO2 group; 156.1 +/- 15.1 and 269.6 +/- 45.2% in the cisplatin group; and 118.3 +/- 28.1 and 210.3 +/- 55.1% in the combined group. RTV was significantly lower in the CO2 group than in the control group (day 3; P