A chitinase with antifungal activity from naked oat (Avena chinensis) seeds
A chitinase with antifungal activity from naked oat (Avena chinensis) seeds
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一种来自裸燕麦 (Avena chinensis) 种子的具有抗真菌活性的几丁质酶。
DOI：
10.1111/jfbc.12713
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发表时间：
2019-02-01
影响因子：
4
通讯作者：
Wang, Zhuanhua
中科院分区：
文献类型：
--
作者：
Li, Chen;Li, Xiaoping;Wang, Zhuanhua
A chitinase was purified from naked oat (Avena chinensis) seeds using simple chromatographic techniques. Its molecular weight and isoelectric point were determined as 35 kDa and 8.9, respectively. The purified chitinase exhibited specific activity of 3.6 U/mg and 15.6% yield using colloidal chitin as substrate. Partial amino acid sequence analysis and homology search indicated that it probably belonged to Class I plant chitinase, glycosyl hydrolase family 19. With chitin as substrate, the optimum pH and temperature of the chitinase were pH 7.0 and 40 degrees C, respectively. The chitinase was remarkably stable from 30 degrees C up to 50 degrees C, but was inactivated at high temperatures above 85 degrees C. Antifungal activity in vitro tests demonstrated this purified chitinase had potent, dose-dependent inhibitory activity against the fungi Panus conchatus and Trichoderma reesei.
