Muscular derivatives of the cranialmost somites revealed by long-term fate mapping in the Mexican axolotl (Ambystoma mexicanum)
Muscular derivatives of the cranialmost somites revealed by long-term fate mapping in the Mexican axolotl (Ambystoma mexicanum)
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DOI：
10.1111/j.1525-142x.2007.00197.x
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发表时间：
2007-11-01
影响因子：
2.9
通讯作者：
Olsson, Lennart
中科院分区：
文献类型：
--
作者：
Piekarski, Nadine;Olsson, Lennart
单个体节的命运还没有从比较的角度与现代细胞标记方法进行分析。我们所知道的大多数是基于鹌鹑小鸡嵌合体的工作。因此，尽管脊椎动物之间存在解剖学差异，但细胞命运在多大程度上得到了保护尚不清楚。我们已经分析了细胞命运的cranialmesitites，重点是体节二，在墨西哥蝾螈（Ambystoma mexicanum）。通过注射葡聚糖-荧光素标记体节细胞，并在石蜡切片中发育2个月后使用免疫荧光检测体节细胞。我们的数据证实并扩展了早期基于蝾螈经典组织学的研究。我们发现，体节2有助于不同的肌肉，骨骼元素，头部和颅躯干区域的结缔组织。第二体节和第三体节的细胞向腹侧迁移，并形成颏舌骨肌和颈直肌。我们提供的证据表明，特定的形成下鳃肌肉组织的体节的腹侧过程可能是可变的，在不同类别的脊椎动物。我们进一步证明，毫米cucullaris和criterator喉，这是早期被认为有一个鳃的起源，从体节材料的方式非常相似的鹌鹑小鸡嵌合体的研究结果。我们的研究结果表明，体节衍生物的模式是高度保守的四足动物。
The fate of single somites has not been analyzed from a comparative perspective with modern cell-marking methods. Most of what we know is based on work using quail-chick chimeras. Consequently, to what degree cell fate has been conserved despite the anatomical differences among vertebrates is unknown. We have analyzed the cell fate of the cranialmost somites, with the focus on somite two, in the Mexican axolotl (Ambystoma mexicanum). Somite cells were marked by injection of dextran-fluorescein and detected using immunofluorescence after 2 months of development in paraffin sections. Our data confirm and extend earlier studies based on classical histology in salamanders. We show that somite two contributes to different muscles, skeletal elements, and connective tissues of the head and cranial trunk region. Cells from somites two and three migrate latero-ventrally and contribute to the hypobranchial muscles mm. geniohyoideus and rectus cervicis. We provide evidence that the specific formation of the hypobranchial musculature from ventral processes of the somites might be variable in different classes of vertebrates. We further demonstrate that mm. cucullaris and dilatator laryngis, which were earlier thought to have a branchial origin, arise from somitic material in a manner very similar to the findings in quail-chick chimeras. Our findings indicate that the pattern of somitic derivatives is highly conserved within tetrapods.