A transient parabiosis skin transplantation model in mice.
A transient parabiosis skin transplantation model in mice.
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DOI：
10.1038/nprot.2012.032
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发表时间：
2012-03-22
期刊：
影响因子：
14.8
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中科院分区：
文献类型：
--
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异种共生--在两只小鼠之间提供共享循环的联合手术--之前已经被开发出来，用于研究造血系统。该方案描述了利用异种繁殖将转基因小鼠的皮肤有效地移植到野生型小鼠身上。它可以用来研究间质细胞在自发性远处癌症扩散(转移)模型中的作用。我们最近发现，原发肿瘤来源的间质细胞可能通过在继发部位提供临时间质而促进转移。研究原发肿瘤来源的间质细胞的作用需要区分和靶向来自原发肿瘤的间质细胞和转移部位的间质细胞。异种生物也可用于研究肿瘤从一种异种生物向另一种异种生物的转移，或研究循环中的炎性细胞或干细胞的作用，从而利用异种生物之间的共享循环。使用该模型研究基质细胞在转移中的作用通常需要长达11周。
Parabiosis—conjoined surgery to provide a shared circulation between two mice—has been previously developed to study the hematopoietic system. This protocol describes the use of parabiosis for efficient transplantation of skin from a transgenic to a wild-type mouse. It can be used to study the role of stromal cells in a spontaneous model of distant cancer dissemination (metastasis). We have recently shown that primary tumor-derived stromal cells may facilitate metastasis by providing a provisional stroma at the secondary site. Studying the role of primary tumor–derived stroma cells requires methods for distinguishing and targeting stromal cells originating from the primary tumor versus their counterparts in the metastatic site. Parabiosis may also be used, taking advantage of the shared circulation between the parabiosed mice, to study tumor metastasis from one parabiont to another, or to investigate the role of circulating inflammatory cells or stem cells. Studying the role of stromal cells in metastasis using this model typically takes up to 11 weeks.