IMMUNE CELL CIRCULATING SUBSETS ARE AFFECTED BY GONADAL-FUNCTION
IMMUNE CELL CIRCULATING SUBSETS ARE AFFECTED BY GONADAL-FUNCTION
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DOI：
10.1016/0024-3205(94)00508-7
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发表时间：
1994-01-01
期刊：
影响因子：
6.1
通讯作者：
COSTANTINI, S
中科院分区：
文献类型：
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作者：
GIGLIO, T;IMRO, MA;COSTANTINI, S
性激素对免疫系统活动的影响已被广泛报道。生育能力的女性比男性更容易患自身免疫性疾病，但这种增加的易感性在绝经后消失。荷尔蒙的变化很可能是造成这一事件的原因，但性荷尔蒙水平和免疫功能之间的精确相关性尚未确定。为此，我们分析了 35 名绝经期女性的外周血淋巴细胞 (PBL) 的表型和自然细胞毒性，将其与 28 名育龄女性和 8 名绝经后女性的相同参数进行比较，并将它们与每组的激素模式相关联。我们还考虑了 8 名过早绝经的女性。通过放射免疫测定检测激素水平，同时通过免疫荧光和 FACS 分析研究 PBL 表型。自然杀伤 (NK) 细胞活性是根据铬释放测定计算的。与育龄女性（2081+/-200 个细胞/mmc）相比，绝经后女性的淋巴细胞总数（1650+/-215 个细胞/mmc）减少，P
Influence on the immune system activity by sex hormones has been widely reported. Fertile women are proner to the onset of autoimmune diseases than men, but this increased susceptibility disappears after menopause. The hormonal changes are very likely to be responsible for this event, but precise correlations between sex hormone levels and immune functions have not been defined. For this reason we have analyzed phenotype and natural cytotoxicity of peripheral blood lymphocytes (PBL) from 35 women in menopause, comparing them with the same parameters of 28 fertile and 8 postmenopausal women and correlating them with the hormonal pattern of each group. We have also considered 8 women with premature menopause. Hormonal levels have been detected by radioimmune assays, while PBL phenotype has been studied by immunofluorescence and FACS analysis. The natural killer (NK) cell activity has been calculated on the basis of a chromium release assay. Postmenopausal women showed a reduction of the number of total lymphocytes (1650+/-215 cells/mmc) in comparison to fertile women (2081+/-200 cells/mmc, P