High throughput detection of capillary stalling events with Bessel beam two-photon microscopy.
High throughput detection of capillary stalling events with Bessel beam two-photon microscopy.
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DOI：
10.1117/1.nph.10.3.035009
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发表时间：
2023-07
期刊：
影响因子：
5.3
通讯作者：
Chen, Ichun A.
中科院分区：
文献类型：
--
作者：
Giblin, John;Kura, Sreekanth;Nunuez, Juan Luis Ugarte;Zhang, Juncheng;Kureli, Gulce;Jiang, John;Boas, David A.;Chen, Ichun A.
毛细血管流动的短暂中断，通常被称为毛细血管“失速”，最近由于其在中断脑血流和氧气输送中的潜在作用而引起了人们的兴趣。研究这种现象的方法受到有限的体积成像速率和繁琐的手动分析的阻碍。精确和有效地量化这些事件的动力学的能力将是理解它们在中风和神经退行性疾病（如阿尔茨海默病）中的潜在作用的关键。我们的研究旨在证明贝塞尔光束双光子显微镜提供的快速体积成像速率与改进的数据分析吞吐量相结合，可以更快，更精确地测量毛细血管失速动态。我们发现，虽然我们的分析方法无法实现完全自动化，但我们能够将分析时间缩短一半，同时还发现了传统盲目手动分析中遗漏的失速事件。所得数据表明，我们的贝塞尔光束系统捕获更多的失速事件相比，光学相干断层扫描，特别是较短的失速事件。然后，我们比较了年轻和老年小鼠组之间失速动力学的差异，以及中风光血栓模型前后失速的变化。最后，我们还展示了监测微动脉动力学以及失速动力学的能力。贝塞尔光束双光子显微镜结合高通量分析是一个强大的工具，研究毛细管失速，由于其能够同时监测数百个毛细管在高帧速率。
Brief disruptions in capillary flow, commonly referred to as capillary “stalling,” have gained interest recently for their potential role in disrupting cerebral blood flow and oxygen delivery. Approaches to studying this phenomenon have been hindered by limited volumetric imaging rates and cumbersome manual analysis. The ability to precisely and efficiently quantify the dynamics of these events will be key in understanding their potential role in stroke and neurodegenerative diseases, such as Alzheimer’s disease. Our study aimed to demonstrate that the fast volumetric imaging rates offered by Bessel beam two-photon microscopy combined with improved data analysis throughput allows for faster and more precise measurement of capillary stall dynamics. We found that while our analysis approach was unable to achieve full automation, we were able to cut analysis time in half while also finding stalling events that were missed in traditional blind manual analysis. The resulting data showed that our Bessel beam system was captured more stalling events compared to optical coherence tomography, particularly shorter stalling events. We then compare differences in stall dynamics between a young and old group of mice as well as a demonstrate changes in stalling before and after photothrombotic model of stroke. Finally, we also demonstrate the ability to monitor arteriole dynamics alongside stall dynamics. Bessel beam two-photon microscopy combined with high throughput analysis is a powerful tool for studying capillary stalling due to its ability to monitor hundreds of capillaries simultaneously at high frame rates.
影响因子：
5.3
作者：
Reeson, Patrick;Schager, Ben;Van Sprengel, Myrthe;Brown, Craig E.
通讯作者：
Brown, Craig E.