Increased formation of reactive oxygen species after permanent and reversible middle cerebral artery occlusion in the rat
Increased formation of reactive oxygen species after permanent and reversible middle cerebral artery occlusion in the rat
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DOI：
10.1097/00004647-199802000-00011
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发表时间：
1998-02-01
影响因子：
6.3
通讯作者：
Dirnagl, U
中科院分区：
文献类型：
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作者：
Peters, O;Back, T;Dirnagl, U
在巴比妥酸盐麻醉的大鼠中，我们通过管腔内线诱导3小时的永久性大脑中动脉闭塞（MCAO）（n = 6），或1小时的MCAO随后2小时的再灌注（n = 6）。通过在顶叶皮层上的闭合颅窗，使用在线体内化学发光（CL）测量梗死边缘中活性氧（ROS）的产生，同时监测梗死周围去极化（PID）的出现。ROS和直流（DC）电位测量的边界区定位在使用激光多普勒扫描的附加实验中得到证实，永久性（n = 5）或可逆性（n = 5）MCAO后通过颅窗绘制区域CBF变化。CL测量显示血管闭塞后ROS形成的短时间（10 - 30分钟）减少，随后从永久性MCAO 100分钟开始显著增加（至162 +/- 51%;基线= 100%; P <0.05）。MCAO 1小时后再灌注导致ROS产生的爆发样模式（峰值：489 +/- 330%：P <0.05）。当实验在诱导MCAO后3小时终止时，永久性和可逆性MCAO后CL仍显著高于基线（分别为190 +/- 67%和211 +/-64%; P <0.05）。在永久性MCAO后的第一个小时，同步DC电位记录检测到6.4 +/- 2.7 PID，第二个小时为4.7 +/- 2.3，第三个小时为2.8 +/- 2.0。在可逆性MCAO的动物中，PID从IS分钟再循环开始被消除。ROS的产生和梗死周围DC电位的变化之间没有时间关系。总之，使用高时间分辨率的ROS检测技术（CL），我们发现，永久性MCAO（初始减少后）伴随着稳定增加的ROS生产在3小时的观察期内，而再灌注后1小时的MCAO产生的ROS形成的爆发。两种ROS产生模式均与PID的发生无关。
In barbiturate-anesthetized rats, we induced 3 hours of permanent middle cerebral artery occlusion (MCAO) by an intraluminal thread (n = 6), or I hour MCAO followed by 2 hours of reperfusion (n = 6). Through a closed cranial window over the parietal cortex, the production of reactive oxygen species (ROS) was measured in the infarct border using online in vivo chemiluminescence (CL) while monitoring the appearance of peri-infarct depolarizations (PID). The border-zone localization of the ROS and direct current (DC) potential measurements was confirmed in additional experiments using laser-Doppler scanning, mapping regional CBF changes through the cranial window after permanent (n = 5) or reversible (n = 5) MCAO. CL measurements revealed a short period (10 to 30 minutes) of reduced ROS formation after vessel occlusion, followed by a significant increase (to 162 +/- 51%; baseline = 100%; P < .05) from 100 minutes of permanent MCAO onward. Reperfusion after a I-hour period of MCAO led to a burst-like pattern of ROS production (peak: 489 +/- 330%: P < .05). When the experiments were terminated 3 hours after induction of MCAO, CL was still significantly increased above baseline after permanent and reversible MCAO (to 190 +/- 67%; and 211 +/- 64%, respectively; P < .05). Simultaneous DC potential recordings detected 6.4 +/- 2.7 PID in the first, 4.7 +/- 2.3 in the second, and 2.8 +/- 2.0 in the third hour after permanent MCAO. In animals with reversible MCAO, PID were abolished from IS-minutes recirculation onward. There was no temporal relationship between ROS production and peri-infarct DC potential shifts. In conclusion, using a high temporal resolution ROS detection technique (CL), we found that permanent MCAO (after an initial decrease) was accompanied by a steady increase of ROS production during the 3-hour observation period, while reperfusion after 1 hour of MCAO produced a burst in ROS formation. Both patterns of ROS production were not related to the occurrence of PID.