Efficiency and cytotoxicity of resin-based desensitizing agents.
Efficiency and cytotoxicity of resin-based desensitizing agents.
复制标题
树脂脱敏剂的效率和细胞毒性。
DOI：
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复制
发表时间：
2002
影响因子：
1.4
通讯作者：
J. Franquin
中科院分区：
文献类型：
--
作者：
J. Camps;I. About;B. Van Meerbeek;J. Franquin
目的
比较五种树脂基脱敏剂在体外降低人牙本质渗透性的效果，并比较它们的细胞毒性。测试的假设是，它们不同的固化技术导致效率和细胞毒性的变化。
材料和方法
从人第三磨牙（每组10个）制备牙本质切片（0.5 +/-0.05mm厚），并在应用一种脱敏剂之前和之后用Flodec装置记录它们的水力传导率。研究了六种脱敏剂：一种光固化剂（Seal and Protect）;一种自固化剂（Pain Free）;不含任何聚合引发剂的基于树脂的试剂（Health-Dent、Gluma脱敏剂、Isodan）;一种基于琥珀酸酯的试剂用作对照（Protect）。对L 929成纤维细胞进行MTT测定，以测量施加到另外的牙本质切片（每组10个）上的六种脱敏剂的细胞毒性。
结果
所有脱敏剂均导致牙本质渗透性大幅度降低。Gluma脱敏剂、Isodan、Pain Free和Protect的效果最好。材料间差异有统计学意义（P = 0.001）。所有材料均无细胞毒性。细胞活力范围从Seal和Protect的88%到Isodan的100%。它们的细胞毒性无差异。
PURPOSE
To compare in vitro the efficacy of five resin-based desensitizing agents at reducing human dentin permeability and to compare their cytotoxicity. The tested hypothesis was that their different curing techniques cause variations in efficiency and cytotoxicity.
MATERIALS AND METHODS
Dentin slices (0.5 +/- 0.05 mm thick) were prepared from human third molars (10 per group) and their hydraulic conductance was recorded before and after application of one of the desensitizing agents with a Flodec device. Six desensitizing agents were studied: one light curing agent (Seal and Protect); one self-curing agent (Pain Free); the resin-based agents without any polymerization initiator (Health-Dent, Gluma Desensitizer, Isodan); one oxalate-based agent served as a control (Protect). A MTT assay on L 929 fibroblasts was performed to measure the cytotoxicity of the six desensitizing agents applied onto additional dentin slices (10 per group).
RESULTS
All the desensitizing agents resulted in a large decrease in dentin permeability. The best results were obtained with Gluma Desensitizer, Isodan, Pain Free and Protect. A statistically significant difference was found among the materials (P = 0.001). All the materials were non-cytotoxic. Cell viability ranged from 88% for Seal and Protect to 100% for Isodan. No difference was found among their cytotoxicity.