Stage-specific insulin binding in mouse preimplantation embryos.
Stage-specific insulin binding in mouse preimplantation embryos.
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DOI：
10.1016/0012-1606(86)90063-1
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发表时间：
1986-07
影响因子：
2.7
通讯作者：
I. Rosenblum;B. A. Mattson;S. Heyner
中科院分区：
文献类型：
--
作者：
I. Rosenblum;B. A. Mattson;S. Heyner
通过使用抗胰岛素抗体的间接免疫荧光技术证明了发育中小鼠胚胎中的阶段特异性胰岛素结合。浓度依赖性的荧光标记，观察到在桑椹胚和囊胚发育阶段，而没有反应，在未受精的卵母细胞或2-，4-和8-细胞胚胎。这些意见的可能意义进行了讨论。这是第一次报告的阶段特异性胰岛素结合在哺乳动物植入前的发展。
Stage-specific insulin binding in the developing mouse embryo was demonstrated by an indirect immunofluorescence technique using an antibody against insulin. Concentration-dependent fluorescence labeling was observed in the morula and blastocyst stages of development, whereas no reactivity was seen in unfertilized oocytes or in 2-, 4-, and 8-cell embryos. The possible significance of these observations is discussed. This represents the first report of stage-specific insulin binding during mammalian preimplantation development.