Generation of natural killer cells from bone marrow precursors in vitro.
Generation of natural killer cells from bone marrow precursors in vitro.
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在体外从骨髓前体生成自然杀伤细胞。
DOI：
10.4049/jimmunol.134.2.802
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发表时间：
1986
期刊：
影响因子：
6.4
通讯作者：
T. Kalland
中科院分区：
文献类型：
--
作者：
T. Kalland
描述了一种简单且高度可重复的骨髓培养系统，用于从骨髓中的未成熟前体产生具有细胞溶解活性的NK细胞。NK细胞可以用各种来源的IL-2产生，包括Con A条件培养基、产生IL-2的细胞系的上清液和重组IL-2。IL-1、IL-3和α/β干扰素在骨髓细胞中均未诱导显著的细胞毒性。基于细胞毒性细胞的表型特征（aGM 1+、Thy 1 +/-、Ly 1-2- X Ia-、RIL-2 +/-、H2+）及其靶标特异性谱，将细胞毒性细胞鉴定为NK细胞。有意添加外周血单核细胞作为成熟NK细胞的来源，并消除表达成熟NK细胞特异性标志物的细胞，表明生成的NK细胞是骨髓中NK细胞前体的后代，而不是来源于污染骨髓制剂的成熟NK细胞。因此，表明不仅功能活性NK细胞而且其前体细胞的分化和生长高度依赖于IL-2。这种培养系统应有助于研究NK细胞与其他细胞谱系的起源以及NK细胞从其前体成熟的调节。
A simple and highly reproducible bone marrow culture system for the generation of cytolytically active NK cells from immature precursors in the bone marrow is described. The NK cells can be generated with various sources of IL-2, including Con A-conditioned medium, supernatants from IL-2-producing cell lines and recombinant IL-2. Neither IL-1, IL-3 nor alpha/beta interferon induced significant cytotoxicity in bone marrow cells. Identification of the cytotoxic cells as NK cells was based on their phenotypic characteristics (aGM1+, Thy 1 +/-, Ly 1-2- X Ia-, RIL-2 +/-, H2+), as well as their spectrum of target specificity. The deliberate addition of peripheral blood mononuclear cells as a source of mature NK cells and elimination of cells expressing markers specific for mature NK cells indicated that the generated NK cells were descendants of precursors of NK cells harboured in the bone marrow and not derived from mature NK cells contaminating the bone marrow preparations. Thus, it was shown that not only functionally active NK cells but also their precursors are highly dependent on IL-2 for differentiation and growth. This culture system should be helpful in studying the origin of NK cells in relation to other cell lineages as well as the regulation of the maturation of NK cells from their precursors.