Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway
Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway
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DOI：
10.1016/j.peptides.2010.08.003
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发表时间：
2010-11-01
期刊：
影响因子：
3
通讯作者：
Zhao Xiuyun
中科院分区：
文献类型：
--
作者：
Qi Gaofu;Zhu Fayin;Zhao Xiuyun
解淀粉芽孢杆菌WH 1通过产生一种新的表面活性素来抑制真菌的生长，这种表面活性素被称为WH 1 fungin。WH 1 fungin通过两种模式发挥抗真菌作用：高浓度引起细胞膜穿孔和低浓度诱导细胞凋亡。WH 1 fungin还能抑制葡聚糖合成酶，导致真菌细胞壁胼胝质合成减少。结果表明，经WH 1处理后，真菌细胞内存在ROS积累、PS外化、DNA链断裂和caspase样活性。结果表明，它不能抑制而能增强WH 1 fungin诱导的真菌细胞凋亡，经亲和层析分离后，发现WH 1 fungin与线粒体膜上的ATP酶结合并导致真菌细胞中ATP酶活性降低。标记的WH 1真菌素，其可以结合到线粒体膜上，在真菌细胞中显示绿色荧光。之后，细胞色素C从线粒体释放，然后细胞色素C与半胱氨酸蛋白酶9作用，通过细胞内途径诱导凋亡。细胞外途径也可能是WH1真菌素诱导细胞凋亡的原因。综上所述，我们报告脂肽可以诱导真菌细胞凋亡，脂肽诱导细胞凋亡可能是芽孢杆菌在自然栖息地的常见抗真菌机制（C）2010 Elsevier Inc版权所有
Bacillus amyloliquefaciens WH1 inhibit the growth of fungi by producing a new surfactin called as WH1fungin WH1fungin plays an anti-fungal role by two models high concentration to elicit pores on cell membrane and low concentration to induce apoptosis WH1fungin can also inhibits the glucan synthase resulting in a decreased synthesis of callose on fungal cell wall Further detection revealed that classical apoptotic markers such as reactive oxygen species (ROS) accumulation phosphatidylserine (PS) externalization DNA strand breaks and caspase-like activities could be found in fungal cells after treated by WH1fungin Oligomycin was used as an inhibitor to block the mitochondria-dependent apoptosis in fungal cells and results showed it could not inhibit but enhance the apoptosis induced by WH1fungin After isolation by affinity chromatography WH1fungin was found to bind with ATPase on the mitochondrial membrane and result in a decreased ATPase activity in fungal cells This was further verified by treating fungal cells with FITC-labeled WH1fungin which could bind to the mitochondrial membrane showing green fluorescence in fungal cells After that cytochrome C was released from the mitochondria which then acted with caspase 9 to induce apoptosis by an intracellular pathway High caspase 8 activity was also detectable in apoptotic fungal cells indicating that an extracellular pathway might also be responsible for apoptosis induced by WH1fungin Taken together we report that lipopeptide can induce apoptosis in fungal cells and induction of apoptosis by lipopeptide might be a common anti-fungal mechanism of Bacillus in the natural habitat (C) 2010 Elsevier Inc All rights reserved