Acute and chronic alcohol dose: population differences in behavior and neurochemistry of zebrafish.
Acute and chronic alcohol dose: population differences in behavior and neurochemistry of zebrafish.
复制标题
DOI：
10.1111/j.1601-183x.2009.00488.x
复制
发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Krishnannair R
中科院分区：
文献类型：
--
作者：
Gerlai R;Chatterjee D;Pereira T;Sawashima T;Krishnannair R
几十年来，斑马鱼一直处于发育遗传学的前沿，在神经行为遗传学方面也越来越受到关注。有人提出用酒精引起的人类大脑功能和行为的变化来建模。在这里，成年斑马鱼种群AB和SF（短鳍野生型）暴露于慢性治疗（在0.00%或0.50%酒精浓度中数天）和随后的急性治疗（在0.00%、0.25%、0.50%或1.00%酒精浓度中1小时）。斑马鱼对电脑动画图像的行为反应，包括斑马鱼鱼群和捕食者，使用视频跟踪进行量化。采用高精度液相色谱电化学检测法测定了鱼脑内多巴胺能和血清素能系统的神经化学变化。研究结果显示，在酒精引起的变化的许多方面存在遗传差异，包括首次戒酒的行为影响和对酒精的神经化学反应。例如，戒除酒精可以消除AB鱼的浅滩，增加多巴胺和3,4-二羟基苯乙酸，但SF鱼没有。研究结果表明，首先，急性和慢性酒精引起的变化是可量化的自动化行为范式；其次，强大的神经化学变化也可以检测到；第三，遗传因素影响酒精引起的行为和神经递质水平的变化。尽管在这一点上，酒精引起的行为和神经化学变化背后的因果关系是推测性的，但结果表明，斑马鱼将是分析成人大脑中酒精引起的功能变化的生物学机制的有用工具。
The zebrafish has been in the forefront of developmental genetics for decades and has also been gaining attention in neurobehavioral genetics. It has been proposed to model alcohol-induced changes in human brain function and behavior. Here, adult zebrafish populations, AB and SF (short-fin wild type), were exposed to chronic treatment (several days in 0.00% or 0.50% alcohol v/v) and a subsequent acute treatment (1 h in 0.00%, 0.25%, 0.50% or 1.00% alcohol). Behavioral responses of zebrafish to computer-animated images, including a zebrafish shoal and a predator, were quantified using videotracking. Neurochemical changes in the dopaminergic and serotoninergic systems in the brain of the fish were measured using high-precision liquid chromatography with electrochemical detection. The results showed genetic differences in numerous aspects of alcohol-induced changes, including, for the first time, the behavioral effects of withdrawal from alcohol and neurochemical responses to alcohol. For example, withdrawal from alcohol abolished shoaling and increased dopamine and 3,4-dihydroxyphenylacetic acid in AB but not in SF fish. The findings show that, first, acute and chronic alcohol induced changes are quantifiable with automated behavioral paradigms; second, robust neurochemical changes are also detectable; and third, genetic factors influence both alcohol-induced behavioral and neurotransmitter level changes. Although the causal relationship underlying the alcohol-induced changes in behavior and neurochemistry is speculative at this point, the results suggest that zebrafish will be a useful tool for the analysis of the biological mechanisms of alcohol-induced functional changes in the adult brain.