RECOGNITION OF IDIOTYPES IN LYMPHOCYTE INTERACTIONS .1. IDIOTYPIC SELECTIVITY IN THE COOPERATION BETWEEN LYMPHOCYTE-T AND LYMPHOCYTE-B
RECOGNITION OF IDIOTYPES IN LYMPHOCYTE INTERACTIONS .1. IDIOTYPIC SELECTIVITY IN THE COOPERATION BETWEEN LYMPHOCYTE-T AND LYMPHOCYTE-B
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DOI：
10.1002/eji.1830081205
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发表时间：
1978-01-01
影响因子：
5.4
通讯作者：
EICHMANN, K
中科院分区：
文献类型：
--
作者：
HETZELBERGER, D;EICHMANN, K
在体外证明了T [胸腺衍生]和B [骨髓衍生]淋巴细胞之间的合作具有对A组链球菌碳水化合物的特异性的独特型限制。在体内用针对A5 A独特型的抗独特型抗体[Ab]致敏并且为A5 A独特型阳性的T辅助细胞仅与A5 A独特型阳性B细胞合作，即使存在A5 A独特型阳性和A5 A独特型阴性B细胞的混合物。在用抗A5 A独特型Ab体内抑制后用A组链球菌疫苗体内致敏的A5 A独特型阴性T辅助细胞不能与用抗A5 A独特型Ab致敏且为A5 A独特型阳性的B细胞合作。A5 A独特型阴性和A5 A独特型阳性T细胞的混合物与A5 A独特型阴性和A5 A独特型阳性B细胞协同作用。在基因VH [H链可变区]相同的T细胞和B细胞的合作与基因VH不同的T细胞和B细胞的合作进行比较的实验中，不能证明T细胞和B细胞分别识别载体和半抗原决定簇的独特型限制。T和B细胞之间的通信的各种模型进行了讨论。对于成功的T-B合作，可能需要2种类型的T辅助细胞，1种识别抗原，另一种识别B细胞的独特型。
Idiotypic restrictions are demonstrated in vitro for the cooperation between T [thymus-derived] and B [bone marrow-derived] lymphocytes with specificity for Group A streptococcal carbohydrate. T helper cells which were primed in vivo with anti-idiotypic antibodies [Ab] to the A5A idiotype and are A5A idiotype-positive, cooperate only with A5A idiotype-positive B cells, even when mixtures of A5A idiotype-positive and A5A idiotype-negative B cells are present. A5A idiotype-negative T helper cells that were primed in vivo with Group A streptococcal vaccine after in vivo suppression with anti-A5A idiotypic Ab are unable to cooperate with B cells which were primed with anti-A5A idiotype Ab and are A5A idiotype-positive. Mixtures of A5A idiotype-negative and A5A idiotype-positive T cells cooperate with A5A idiotype-negative and A5A idiotype-positive B cells. Idiotypic restrictions could not be demonstrated for T and B cells recognizing carrier and hapten determinants, respectively, in experiments in which the cooperation of genetically VH [H chain variable region]-identical T and B cells was compared to the cooperation of genetically VH-different T and B cells. Various models for the communication between T and B cells are discussed. For successful T-B cooperation, 2 types of T helper cells may be required, 1 recognizing the antigen and the other recognizing the idiotype of the B cell.