Decomposition of DNA Nucleobases by Laser Irradiation of Gold Nanoparticles Monitored by Surface-Enhanced Raman Scattering
Decomposition of DNA Nucleobases by Laser Irradiation of Gold Nanoparticles Monitored by Surface-Enhanced Raman Scattering
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DOI：
10.1021/acs.jpcc.5b10564
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发表时间：
2016-01
影响因子：
3.7
通讯作者：
R. Schürmann;I. Bald
中科院分区：
文献类型：
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作者：
R. Schürmann;I. Bald
已经提出了不同的方法来治疗癌细胞，使用金纳米粒子（AuNPs）结合从红外激光到高能离子束的辐射。在这里，我们研究了DNA/RNA核碱基胸腺嘧啶（T）和尿嘧啶（U）和众所周知的放射增敏剂5-溴尿嘧啶（BrU）在金纳米粒子附近的分解，这是用纳秒脉冲激光（532 nm）照射匹配的表面等离子体共振的金纳米粒子。通过紫外可见吸收光谱和表面增强拉曼散射（Sers）分析了碱基的诱导损伤。在激光照射下观察到明显的DNA损伤。Sers光谱表明，断裂的芳环系统的T和U的主要形式的损害，而与BrU主要的Br-C键的裂解和形成Br-离子观察。这伴随着BrU向U的部分转化。所观察到的损伤至少部分归因于中间形成的低能电子从的…
Different approaches have been proposed to treat cancer cells using gold nanoparticles (AuNPs) in combination with radiation ranging from infrared lasers to high-energy ion beams. Here we study the decomposition of the DNA/RNA nucleobases thymine (T) and uracil (U) and the well-known radiosensitizer 5-bromouracil (BrU) in close vicinity to AuNPs, which are irradiated with a nanosecond pulsed laser (532 nm) matching the surface plasmon resonance of the AuNPs. The induced damage of nucleobases is analyzed by UV–vis absorption spectroscopy and surface-enhanced Raman scattering (SERS). A clear DNA damage is observed upon laser irradiation. SERS spectra indicate the fragmentation of the aromatic ring system of T and U as the dominant form of damage, whereas with BrU mainly the cleavage of the Br–C bond and formation of Br– ions is observed. This is accompanied by a partial transformation of BrU into U. The observed damage is at least partly ascribed to the intermediate formation of low-energy electrons from th...