Properties of a factor increasing monocytopoiesis (FIM) occurring in serum during the early phase of an inflammatory reaction.
Properties of a factor increasing monocytopoiesis (FIM) occurring in serum during the early phase of an inflammatory reaction.
复制标题
炎症反应早期阶段血清中出现的增加单核细胞生成 (FIM) 的因子的特性。
作者：
D. van Waarde;E. Hulsing;R. van Furth
在腹腔注射聚苯乙烯乳胶颗粒引起的急性炎症反应发作期间，增加单核细胞增生（FIM）的因素已被证明。它本质上是蛋白质，不含其功能所必需的碳水化合物部分，很可能不是糖蛋白。FIM的分子量在18000 - 24000道尔顿之间（用超滤膜和Sephadex G100凝胶过滤测定）。FIM诱导的单核细胞增多是剂量依赖性的。FIM具有耐热性，在血清中37℃时半衰期约为20分钟；加入-氨基己酸可延缓温度失活，在37℃时的半衰期约为45 min。在体外用炎症反应诱导剂处理正常小鼠血液时，血清中不产生FIM活性。FIM对巨噬细胞没有趋化活性，不是一种凝血因子，不是补体系统的生物活性片段，在体外骨髓集落试验中没有集落刺激或增强活性。在这些结果的基础上，假设了一种单核细胞生成的体液调节机制。
A factor increasing monocytopoiesis (FIM) has been demonstrated during the onset of an acute inflammatory reaction caused by an intraperitoneal injection of polystyrene latex particles. It is protein in nature, does not contain a carbohydrate moiety essential for its function, and is very probably not a glycoprotein. The molecular weight of FIM lies between 18,000 and 24,000 daltons (determined with both ultrafiltration membranes and gel filtration on Sephadex G100). The monocytosis induced by FIM is dose dependent. FIM is thermolabile, having a half-time of about 20 min at 37 degrees C in serum; temperature inactivation can be delayed by the addition of epsilon-aminocaproic acid, the half-time at 37 degrees C then being about 45 min. In vitro treatment of normal murine blood with the inducers of the inflammatory reaction does not result in FIM activity in the serum. FIM dose not have chemotactic activity toward macrophages, is not a clotting factor, is not a biologically active fragment of the complement system, and has no colony-stimulating or-enhancing activity in the vitro bone marrow colony assay. On the basis of these results, a mechanism is postulated for the humoral regulation of monocytopoiesis.