GM1b is a new member of antigen for serum antibody in Guillain-Barre syndrome
GM1b is a new member of antigen for serum antibody in Guillain-Barre syndrome
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DOI：
10.1212/wnl.47.1.237
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发表时间：
1996-07-01
期刊：
影响因子：
9.9
通讯作者：
Kanazawa, I
中科院分区：
文献类型：
--
作者：
Kusunoki, S;Iwamori, M;Kanazawa, I
一些格林-巴利综合征患者的血清抗体识别人脑单唾液神经节苷脂部分中的一种次要成分的抗原。我们用医用珠柱层析和制备性TLC纯化了该抗原，该抗原在薄层层析(TLC)上的迁移位置略低于GM1。包括快原子轰击质谱仪在内的结构分析表明，它就是GM1b。用纯化的GM1b进行酶联免疫吸附试验(EL ISA)，在104例受检者中有22例抗GM1b抗体阳性，而其他疾病对照组和正常对照组血清中均未检测到抗GM1b抗体。在检测的11种神经节苷脂抗原中，有4种血清仅识别GM1b。其余18份血清均有针对其他抗原的抗体，其中大部分与GM1b没有共同的末端表位。在9份抗GalNAc-GD1a抗体阳性的血清中，有8份也有抗GM1b抗体。抗单唾液酸神经节苷脂GM1b的抗体被发现是格林-巴利综合征的有用诊断标记物。这种抗体是否在该综合征的发病机制中起作用，还需要进一步的研究。
Serum antibody from some patients with Guillain-Barre syndrome recognized an antigen of a minor component in human brain monosialoganglioside fraction. We purified that antigen, which migrated at a position slightly lower than that of GM1 on a thin-layer chromatogram (TLC), by using Iatrobeads column chromatography and preparative TLC. Structural analyses, including fast atom bombardment mass spectrometry, showed it to be GM1b. An enzyme-linked immunosorbent assay (ELISA) using purified GM1b showed that anti-GM1b antibody was present in 22 of 104 cases tested, No anti-GM1b antibody was present in the sera from control patients with other diseases or from the normal controls. Four sera recognized only GM1b among the 11 ganglioside antigens tested. The other 18 sera had antibodies to other antigens, most of which shared no terminal epitope with GM1b. Eight of nine sera samples with anti-GalNAc-GD1a antibody also had anti-GM1b antibody. Antibody to a minor monosialoganglioside, GM1b, was found to be a useful diagnostic marker for Guillain-Barre syndrome. Further study is needed to determine whether this antibody plays a role in the pathogenetic mechanism of the syndrome.