Changes in gonadotropin-releasing hormone in the cerebral ganglion of the Manila clam Ruditapes philippinarum during gonadal development
Changes in gonadotropin-releasing hormone in the cerebral ganglion of the Manila clam Ruditapes philippinarum during gonadal development
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DOI：
10.1016/j.cbpa.2022.111228
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发表时间：
2022-05-10
影响因子：
2.3
通讯作者：
Amano, Masafumi
中科院分区：
文献类型：
--
作者：
Ojima, Daisuke;Hirano-Maeda, Yuki;Amano, Masafumi
促性腺激素释放激素(GnRH)在脊椎动物和无脊椎动物的生殖过程中都起着重要的作用，但对双壳类的性腺发育过程知之甚少。建立了菲律宾蛤仔GnRH(RpGnRH)的时间分辨荧光免疫分析方法，并测定了性腺发育过程中脑神经节(CG)中rpGnRH的含量和血淋巴中性类固醇激素的含量。抗rpGnRH抗体与其他形式的GnRH的交叉反应是
Gonadotropin-releasing hormone (GnRH) plays an important role in reproduction in both vertebrates and invertebrates; however, little is known about GnRH during gonadal development in bivalves. We developed a time resolved fluoroimmunoassay (TR-FIA) for Manila clam Ruditapes philippinarum GnRH (rpGnRH) and measured the amount of rpGnRH in the cerebral ganglion (CG) and sex steroid hormones in the hemolymph during gonadal development. The cross-reactivity of the anti-rpGnRH antibody against other forms of GnRH was