Epileptiform activity in microcultures containing one excitatory hippocampal neuron.
Epileptiform activity in microcultures containing one excitatory hippocampal neuron.
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DOI：
10.1152/jn.1991.65.4.761
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发表时间：
1991-04
影响因子：
2.5
通讯作者：
M. M. Segal-M.
中科院分区：
文献类型：
--
作者：
M. M. Segal-M.
1. 阵发性去极化转变 (PDS) 发生在发作间期癫痫样活动期间。持续去极化是发作活动的特征，并且在持续去极化期间也会发生类似PDS的事件。为了在更简单的背景下研究癫痫样活动的这些要素，我使用了 Furshpan 和 Potter 的体外慢性兴奋性阻滞癫痫模型以及 Segal 和 Furshpan 的微培养技术。 2. 细胞内记录由 93 个单神经元微培养物进行。这些孤立神经元中有 40 个是兴奋性的，当犬尿酸盐从灌注溶液中去除时，它们的动作电位被 PDS 样事件或持续去极化所取代。 PDS 样事件与完整皮层、大量培养物和具有多个神经元的微培养物中的 PDS 相似。在没有记录动作电位的情况下，在孤立的兴奋性神经元中也观察到小电压波动。 40 个兴奋性神经元中有 5 个出现持续去极化。 93 个孤立神经元中有 48 个是抑制性的，在动作电位后出现荷包牡丹碱敏感的超极化（归因于 γ-氨基丁酸-A 自动激活）。没有一个单独的抑制性神经元表现出持续的去极化。 93 个神经元中有 5 个对犬尿酸盐和荷包牡丹碱均不敏感，并且不属于兴奋性或抑制性类别。 3. N-甲基-D-天冬氨酸（NMDA）和非NMDA谷氨酸受体均有助于PDS样事件和持续去极化。对于较小的电压波动，只有非 NMDA 谷氨酸受体成分是明显的。 4. 还从两个神经元微培养物中进行细胞内记录，每个培养物包含一个兴奋性神经元和一个抑制性神经元。在五种微培养物中出现持续去极化，每种情况下仅在兴奋性神经元中出现。
1. Paroxysmal depolarizing shifts (PDSs) occur during interictal epileptiform activity. Sustained depolarizations are characteristic of ictal activity, and events resembling PDSs also occur during the sustained depolarizations. To study these elements of epileptiform activity in a simpler context, I used the in vitro chronic-excitatory-block model of epilepsy of Furshpan and Potter and the microculture technique of Segal and Furshpan. 2. Intracellular recordings were made from 93 single-neuron microcultures. Forty of these solitary neurons were excitatory, their action potentials were replaced by PDS-like events or sustained depolarizations as kynurenate was removed from the perfusion solution. PDS-like events were similar to PDSs in intact cortex, mass cultures, and microcultures with more than one neuron. Small voltage fluctuations were also seen in solitary excitatory neurons in the absence of recorded action potentials. Sustained depolarizations developed in 5 of the 40 excitatory neurons. Forty-eight of the 93 solitary neurons were inhibitory, with bicuculline-sensitive hyperpolarizations after the action potential (ascribable to gamma-aminobutyric acid-A autapses). None of the solitary inhibitory neurons displayed sustained depolarizations. Five of the 93 neurons were insensitive to both kynurenate and bicuculline and were not placed in either the excitatory or the inhibitory category. 3. Both N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptors contributed to the PDS-like events and sustained depolarizations. Only a non-NMDA glutamate receptor component was evident for the small voltage fluctuations. 4. Intracellular recordings were also made from two-neuron microcultures, each containing one excitatory neuron and one inhibitory neuron. Sustained depolarizations developed in five microcultures, in each case only in the excitatory neuron.