Molecular analysis of human and bovine tubercle bacilli from a local setting in Nigeria
Molecular analysis of human and bovine tubercle bacilli from a local setting in Nigeria
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DOI：
10.1128/jcm.44.1.29-34.2006
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发表时间：
2006-01-01
影响因子：
9.4
通讯作者：
Gordon, SV
中科院分区：
文献类型：
--
作者：
Cadmus, S;Palmer, S;Gordon, SV
为了建立尼日利亚引起结核病的菌株的分子流行病学基线，对来自人类和牛的分离株进行了调查。spoligo分型和可变数串联重复分析显示，尼日利亚西南部lbadan的大多数人类结核病是由一组密切相关的结核分枝杆菌菌株引起的。通过缺失分型，我们发现该样本中大约13%的人类疾病是由非洲分枝杆菌和牛分枝杆菌菌株引起的，而不是结核分枝杆菌。对从尼日利亚牛身上恢复的牛分枝杆菌菌株的分子分析表明，它们形成了一组密切相关的菌株，与邻国喀麦隆的菌株相似。令人惊讶的是，从人类身上恢复的牛分枝杆菌菌株与牛株的分子类型不匹配，并讨论了可能的原因。这是对尼日利亚人与牛之间流行的结核分枝杆菌复合菌株进行的首次分子分析，其结果对疾病控制具有重要意义。
To establish a molecular epidemiological baseline for the strains causing tuberculosis in Nigeria, a survey of isolates from humans and cattle was carried out. Spoligotyping and variable-number tandem-repeat analysis revealed that the majority of tuberculosis disease in humans in lbadan, southwestern Nigeria, is caused by a single, closely related group of Mycobacterium tuberculosis strains. Using deletion typing, we show that approximately 13% of the disease in humans in this sample was caused by strains of Mycobacterium africanum and Mycobacterium bovis rather than M. tuberculosis. Molecular analysis of strains of M. bovis recovered from Nigerian cattle show that they form a group of closely related strains that show similarity to strains from neighboring Cameroon. Surprisingly, the strains of M. bovis recovered from humans do not match the molecular type of the cattle strains, and possible reasons for this are discussed. This is the first molecular analysis of M. tuberculosis complex strains circulating among humans and cattle in Nigeria, the results of which have significant implications for disease control.