Liquid Crystal Elastomer-Based Microelectrode Array for In Vitro Neuronal Recordings.
Liquid Crystal Elastomer-Based Microelectrode Array for In Vitro Neuronal Recordings.
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DOI：
10.3390/mi9080416
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发表时间：
2018-08-20
期刊：
影响因子：
3.4
通讯作者：
Ware TH
中科院分区：
文献类型：
--
作者：
Rihani RT;Kim H;Black BJ;Atmaramani R;Saed MO;Pancrazio JJ;Ware TH
基于聚合物的生物医学电子学提供了一个可调节的平台，可以在体外和体内与神经组织相互作用。最终，控制神经接口功能特性的能力可能为研究神经系统或恢复神经退行性疾病患者的功能提供重要优势。液晶弹性体(LCE)是一类智能材料，当暴露在各种刺激下时，可以可逆地改变形状。我们对LCEs的兴趣是基于利用这种形状变化将电极位置部署在与慢性植入相关的炎症组织区域之外。作为第一步，我们证明了LCEs是一种细胞相容材料，可以用作制备微电极阵列(MEA)的衬底，能够在体外记录单个单位的活动。根据国际标准化组织协议10993-5使用成纤维细胞和原代小鼠皮质神经元进行测定，LCEs的提取物是无细胞毒性的(70%归一化百分比存活率)。将培养在常规微电极阵列上的大脑皮层神经元暴露于LCE提取液48h后，LCEs也没有功能性神经毒性。电化学阻抗谱表明，在LCEs上构建的微电极阵列是稳定的。在1 kHz下对阻抗和相位的检查显示，在磷酸盐缓冲盐水(PBS)中30天的监测中，结果完全在电生理记录的范围内。此外，LCE阵列被证明支持在体外27天内可行的皮质神经元培养，并能够记录与传统商业可获得的微电极阵列相媲美的显著的细胞外生物势。
Polymer-based biomedical electronics provide a tunable platform to interact with nervous tissue both in vitro and in vivo. Ultimately, the ability to control functional properties of neural interfaces may provide important advantages to study the nervous system or to restore function in patients with neurodegenerative disorders. Liquid crystal elastomers (LCEs) are a class of smart materials that reversibly change shape when exposed to a variety of stimuli. Our interest in LCEs is based on leveraging this shape change to deploy electrode sites beyond the tissue regions exhibiting inflammation associated with chronic implantation. As a first step, we demonstrate that LCEs are cellular compatible materials that can be used as substrates for fabricating microelectrode arrays (MEAs) capable of recording single unit activity in vitro. Extracts from LCEs are non-cytotoxic (>70% normalized percent viability), as determined in accordance to ISO protocol 10993-5 using fibroblasts and primary murine cortical neurons. LCEs are also not functionally neurotoxic as determined by exposing cortical neurons cultured on conventional microelectrode arrays to LCE extract for 48 h. Microelectrode arrays fabricated on LCEs are stable, as determined by electrochemical impedance spectroscopy. Examination of the impedance and phase at 1 kHz, a frequency associated with single unit recording, showed results well within range of electrophysiological recordings over 30 days of monitoring in phosphate-buffered saline (PBS). Moreover, the LCE arrays are shown to support viable cortical neuronal cultures over 27 days in vitro and to enable recording of prominent extracellular biopotentials comparable to those achieved with conventional commercially-available microelectrode arrays.
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影响因子：
8.4
作者：
Boothby, Jennifer M.;Kim, Hyun;Ware, Taylor H.
通讯作者：
Ware, Taylor H.
影响因子：
64.8
作者：
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通讯作者：
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影响因子：
5
作者：
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通讯作者：
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影响因子：
12.6
作者：
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通讯作者：
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影响因子：
14.8
作者：
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通讯作者：
Mok, Sue-Ann