Materno-fetal coordination of stress-induced Fos expression in the hypothalamic paraventricular nucleus during pregnancy
Materno-fetal coordination of stress-induced Fos expression in the hypothalamic paraventricular nucleus during pregnancy
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DOI：
10.1016/s0306-4522(02)00781-9
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Nakamura, S
中科院分区：
文献类型：
--
作者：
Fujioka, T;Fujioka, A;Nakamura, S
本研究探讨孕期母体应激是否诱导母体和胎儿下丘脑室旁核(PVN)神经元激活，以及母体应激对胎儿下丘脑和PVN脑源性神经营养因子(BDNF)表达的影响。孕鼠在妊娠第21天暴露于3种不同程度的母体应激(束缚、强迫行走和制动)30min。刺激后30分钟测定母体血浆皮质酮，以评估应激程度。各应激反应组母血皮质酮均升高(制动+GT；强迫行走+束缚)。此外，作为神经元激活标志的Fos蛋白在胎儿和母体PVN中的表达增加，与应激治疗的严重程度直接相关。强迫行走和束缚应激，而不是束缚应激，显著增加了胎儿下丘脑BDNF的表达。这些发现表明，母体应激后胎儿下丘脑-垂体-肾上腺(HPA)的反应反映了母体HPA的激活。此外，脑源性神经营养因子可能在保护胎儿大脑神经元免受严重应激损伤方面发挥作用。(C)2003年IBRO。爱思唯尔科学有限公司出版。版权所有。
This study investigates whether maternal stress during pregnancy induces maternal and fetal hypothalamic paraventricular nucleus (PVN) neuronal activation and the effects of maternal stress on fetal hypothalamic and PVN brain-derived neurotrophic factor (BDNF) expression. Pregnant rats were exposed to three types of maternal stress with varying severity (restraint, forced walking and immobilization) for 30 min on gestational day 21. Severity of stress was assessed by measurement of maternal plasma corticosterone 30 min following the stimulus. Maternal plasma corticosterone increased in each stress response group (immobilization >forced walking>restraint). Further, the expression of Fos protein, a marker of neuronal activation, increased in the fetal and maternal PVN in direct relation to the severity of stress treatments. Forced walking and immobilized stress, but not restraint stress, significantly increased BDNF expression in the fetal hypothalamus.These findings suggest that the fetal hypothalamic-pituitary-adrenal (HPA) response following maternal stress mirrors maternal HPA activation. In addition, BDNF may play a role in protecting fetal brain neurons from damage caused by severe stress. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.