Improved Strategy for Molecular Characterization of African Swine Fever Viruses from Sardinia, Based on Analysis of p30, CD2V and I73R/I329L Variable Regions
Improved Strategy for Molecular Characterization of African Swine Fever Viruses from Sardinia, Based on Analysis of p30, CD2V and I73R/I329L Variable Regions
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DOI：
10.1111/tbed.12504
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发表时间：
2017-08-01
影响因子：
4.3
通讯作者：
Oggiano, A.
中科院分区：
文献类型：
--
作者：
Sanna, G.;Dei Giudici, S.;Oggiano, A.
非洲猪瘟病毒（ASFV）是一种影响猪的高致死性出血性疾病的病原体，对养猪业造成重大经济损失。非洲猪瘟存在于许多非洲国家、几个东欧和中欧国家以及撒丁岛（意大利）。基因组可变区的序列分析已被广泛用于ASFV分离株的分子流行病学研究。先前编码病毒蛋白p54、p72和B602 L基因内的中央可变区（CVR）的基因的测序数据显示，撒丁岛分离株显示出非常低的变异性水平。为了更好地区分这些密切相关的病毒，在这项研究中，我们选择了三个不同的基因组区域来研究基因型内的关系，并提供更准确的评估暴发的起源。从收集在撒丁岛超过13年的时间从ASFV获得的p30和I73 R/I329 L序列的分析证实了显着的遗传稳定性，在这些地区。从1978年到2014年对各种毒株进行的EP 402 R基因（CD 2 v）编码的蛋白质的序列比较显示，撒丁岛病毒在时间上分为两个亚组：一组包括1978年至1990年的历史分离株，第二组由1990年至2014年收集的病毒组成。这些数据，以及从CVR中获得的B602 L基因分析的数据，表明在撒丁岛流行的病毒属于p72基因型I，但自1990年以来在基因组的两个不同区域发生了遗传变异。我们提出了CD 2 v蛋白胞质区作为一个新的遗传标记，可以用来分析来自不同地点的ASFV，以跟踪病毒的传播。我们的研究重申了需要分析其他基因组区域，以改善ASFV的分子特征。
African swine fever virus (ASFV) is the aetiological agent of a highly lethal haemorrhagic disease affecting pigs that inflicts significant economic damage on the swine industry. ASF is present in many African countries, in several eastern and central European countries and in Sardinia (Italy). Sequence analyses of variable genomic regions have been extensively used for molecular epidemiological studies of ASFV isolates. Previous sequencing data of genes that codify for viral protein p54, p72 and the central variable region (CVR) within the B602L gene revealed that Sardinian isolates show a very low level of variability. To achieve a finer level of discrimination among such closely related viruses, in this study, we have chosen three different genome regions to investigate the within-genotype relationships and to provide a more accurate assessment of the origin of outbreaks. The analysis of p30 and I73R/I329L sequences obtained from ASFV collected in Sardinia over a 13-years period confirms a remarkable genetic stability in these regions. The sequence comparison of the protein encoded by the EP402R gene (CD2v), carried out on various strains from 1978 to 2014, revealed a temporal subdivision of Sardinian viruses into two subgroups: one group includes the historical isolates from 1978 to 1990, and the second one is comprised of the viruses collected from 1990 until 2014. These data, together with those obtained from CVR within the B602L gene analysis, demonstrated that the viruses circulating in Sardinia belong to p72 genotype I, but have undergone genetic variations in two different regions of the genome since 1990. We proposed the cytoplasmic region of CD2v protein as a new genetic marker that could be use to analyse ASFVs from different locations to track virus spread. Our study reaffirms the need to analyse other genome regions in order to improve the molecular characterization of ASFV.