Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials.
Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials.
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用于临床试验中人PBMC样品的板内染色、条形码化和冷冻保存的简化质谱细胞术方案。
DOI：
10.1016/j.xpro.2022.101362
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发表时间：
2022-06-17
期刊：
影响因子：
--
通讯作者：
Baginska, Joanna
中科院分区：
文献类型：
--
作者：
Abrecht, Charlotte;Hallisey, Margaret;Dennis, Jenna;Nazzaro, Matthew;Brainard, Martha;Hathaway, Emma;Schork, Abigail N.;Hodi, Stephen;Severgnini, Mariano;Baginska, Joanna
随着大量流式细胞术在临床研究中的广泛应用，需要一种简化和标准化的临床试验中人外周血单个核细胞（PBMC）免疫分型方案。我们提出了一个简化的板内染色方案，适用于多达80个样本，适用于所有质谱细胞术专业水平的实验室，旨在为大型临床队列生成可重现的数据集。在本方案中，我们提供了有关获得有意义结果的要求的详细信息，包括样本质量、条形码和染色样本的批冷冻。可对多达80个待采集样本进行染色、条形码化和冷冻的质谱细胞仪方案冷冻染色、条形码化样本可更好地管理机器采集对门控策略和多参数数据分析的见解适用于所有专业水平的人员，可生成批量效应降低的数据集随着质谱细胞仪在临床研究中的使用越来越多，在临床试验中需要一种用于对人外周血单核细胞（PBMC）进行免疫表型分析的简化和标准化的方案。我们提出了一个简化的板内染色方案，适用于多达80个样本，适用于所有质谱细胞术专业水平的实验室，旨在为大型临床队列生成可重现的数据集。在本方案中，我们提供了有关获得有意义结果的要求的详细信息，包括样本质量、条形码和染色样本的批冷冻。
With the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) in clinical trials is needed. We present a simplified in-plate staining protocol for up to 80 samples, for laboratories of all mass cytometry expertise levels, aimed to generate reproducible datasets for large clinical cohorts. In this protocol, we provide details on the requirements to obtain meaningful results, spanning from sample quality, barcoding, and batch-freezing of stained samples. Mass cytometry protocol to stain, barcode, and freeze up to 80 ready-to-acquire samples Freezing stained, barcoded samples allows for better management of machine acquisition Insights on gating strategy and multiparameter data analysis Useful for all expertise levels to generate datasets with reduced batch effects With the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) in clinical trials is needed. We present a simplified in-plate staining protocol for up to 80 samples, for laboratories of all mass cytometry expertise levels, aimed to generate reproducible datasets for large clinical cohorts. In this protocol, we provide details on the requirements to obtain meaningful results, spanning from sample quality, barcoding, and batch-freezing of stained samples.
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影响因子：
4
作者：
Marsh-Wakefield FM;Mitchell AJ;Norton SE;Ashhurst TM;Leman JK;Roberts JM;Harte JE;McGuire HM;Kemp RA
通讯作者：
Kemp RA
影响因子：
7.3
作者：
Gonder S;Fernandez Botana I;Wierz M;Pagano G;Gargiulo E;Cosma A;Moussay E;Paggetti J;Largeot A
通讯作者：
Largeot A
影响因子：
3.7
作者：
Sumatoh, Hermi R.;Teng, Karen Wei Weng;Newell, Evan W.
通讯作者：
Newell, Evan W.
影响因子：
10.9
作者：
Maecker HT;Harari A
通讯作者：
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