Design of 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde as a reagent for ultrasensitive determination of primary amines by capillary electrophoresis using laser fluorescence detection.
Design of 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde as a reagent for ultrasensitive determination of primary amines by capillary electrophoresis using laser fluorescence detection.
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DOI：
10.1021/ac00005a004
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发表时间：
1991-03
影响因子：
7.4
通讯作者：
J. Liu;Y. Hsieh;D. Wiesler;M. Novotny
中科院分区：
文献类型：
--
作者：
J. Liu;Y. Hsieh;D. Wiesler;M. Novotny
氨基酸和肽，从标准溶液和生物样品，成功地与3-（4-羧基苯甲酰基）-2-喹啉甲醛，在低浓度下，形成高荧光异吲哚衍生物。所形成的混合物被高效毛细管电泳有效地分离，并通过其激光诱导荧光信号检测其成分。在低阿托摩尔（10（-18）mol）范围内达到最小可检测量。
Amino acids and peptides, from both standard solutions and biological samples, were successfully reacted with 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde, at low concentration, to form highly fluorescent isoindole derivatives. The formed mixtures are effectively separated by high-performance capillary electrophoresis and their constituents detected by their laser-induced fluorescence signals. The minimum detectable quantities in the low attomole (10(-18) mol) range are encountered.