Nitrogen-doped carbon quantum dots as an antimicrobial agent against Staphylococcus for the treatment of infected wounds
Nitrogen-doped carbon quantum dots as an antimicrobial agent against Staphylococcus for the treatment of infected wounds
复制标题
氮掺杂碳量子点作为葡萄球菌抗菌剂，用于治疗感染伤口
DOI：
10.1016/j.colsurfb.2019.03.042
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Lin Xinhua
中科院分区：
文献类型：
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作者：
Zhao Chengfei;Wang Xuewen;Wu Lina;Wu Wen;Zheng Yanjie;Lin Liqing;Weng Shaohuang;Lin Xinhua
抗菌药物耐药性日益严重，已成为人类生命健康的潜在危害。需要制造一些针对耐药细菌的新型抗菌物质。随着碳纳米材料的广泛应用和研究，本文通过一步化学路线合成了氮掺杂碳量子点（NCQD）。通过透射电子显微镜 (TEM)、原子力显微镜和动态光散射对 NCQD 的粒径在 2-5 nm 范围内进行了表征。通过紫外可见吸收光谱、X射线光电子能谱和傅里叶变换红外光谱研究了NCQD的官能团和光学性质。纸片扩散试验表明，NCQD 对葡萄球菌具有特异性抗菌活性。 TEM表明NCQD可以破坏金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的细胞结构，但不能对抗大肠杆菌。其抗菌机制可能是带正电荷的NCQD首先与带负电荷的细菌相互作用，然后特异性锚定在葡萄球菌表面的某些特定位点上。 NCQD用于治疗MRSA感染的伤口，显示出与万古霉素相同的治疗效果。苏木精-伊红染色的组织学切片的显微照片显示，NCQD 在有效杀死金黄色葡萄球菌和 MRSA 的浓度下对大鼠主要器官（包括心脏、肝脏、脾脏、肺和肾）造成的毒性可以忽略不计。因此，NCQD 可以开发为一种有前景的葡萄球菌抗菌剂。 NCQD在临床上有望治疗葡萄球菌引起的局部感染，特别是金黄色葡萄球菌和MRSA。
Antimicrobial resistance is becoming more and more serious and has become a potential hazard to human life and health. The fabrication of some new antibacterial substances against resistant bacteria is demanded. With the wide application and research of carbon nanomaterials, nitrogen-doped carbon quantum dots (NCQDs) were synthesized by a one-step chemical route herein. The particle size of NCQDs in the range of 2–5 nm were characterized by transmission electron microscopy (TEM), atomic force microscopy, and dynamic light scattering. The functional groups and optical properties of NCQDs were investigated by UV–vis absorption spectroscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. Disk-diffusion tests showed that the NCQDs had specific antibacterial activity against Staphylococcus. TEM showed that the NCQDs could destroy the cell structure of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) but could not combat Escherichia coli. The antibacterial mechanism may be that positively charged NCQDs firstly interacted with the negatively charged bacteria, and then specifically anchored on some specific sites on the surface of Staphylococcus. The NCQDs were applied to treat wounds infected with MRSA and showed the same therapeutic effect as vancomycin. Photomicrographs of hematoxylin-eosin-stained histological sections showed that the NCQDs at concentrations effectively killing S. aureus and MRSA caused negligible toxicity to the main rat organs, including heart, liver, spleen, lung, and kidney. Thus, the NCQDs can be developed as a promising antibacterial agent for Staphylococcus. And the NCQDs are likely to treat local infections caused by Staphylococcus clinically, especially S. aureus and MRSA.