Influence of Chain Length on the Diffusion and Electrophoresis of DNA Adsorbed on Heterogeneous Supported Lipid Bilayers
Influence of Chain Length on the Diffusion and Electrophoresis of DNA Adsorbed on Heterogeneous Supported Lipid Bilayers
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DOI：
10.1021/la101679n
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发表时间：
2010-08-17
期刊：
影响因子：
3.9
通讯作者：
Kane, Ravi S.
中科院分区：
文献类型：
--
作者：
Athmakuri, Krishna;Rohovie, Marcus;Kane, Ravi S.
该手稿描述了链长对在异质阳离子阳离子支持的脂质双层的吸附的单链DNA（ssDNA）的扩散和电泳的影响。这些研究的激励是由于对开发新型DNA的新策略的兴趣日益激励。我们研究了ssDNA分子的碱基数（n）从21到84不等。荧光后的荧光回收率（FRAP）研究表明，吸附的ssDNA的扩散率（d）与n一样，与n-1相似，类似于n-1先前观察到双链DNA在均匀支持的脂质双层中的扩散。相反，在施加切向电场的存在下，吸附的ssDNA的电泳迁移率独立于N。我们的研究表明，在存在施加的电场的情况下，ssDNA的运动主要是由于电泳，并且没有显着影响。通过电流流。我们的结果还表明，使用不对称的扩散屏障或其他可调障碍物可能有助于对受支持的脂质双层的DNA分离。
This manuscript describes the influence of chain length on the diffusion and electrophoresis of single stranded DNA (ssDNA) adsorbed on heterogeneous cationic supported lipid bilayers. These studies are motivated by the increasing interest in developing novel strategies for the separation of DNA. We studied ssDNA molecules with the number of bases (N) varying from 21 to 84. Fluorescence recovery after photobleaching (FRAP) studies revealed that the diffusivity (D) of adsorbed ssDNA varied with N as D similar to N-1 similar to a trend previously observed for the diffusion of double stranded DNA on homogeneous supported lipid bilayers. In contrast, the electrophoretic mobility of the adsorbed ssDNA in the presence of an applied tangential electric field was independent of N. Our studies indicated that the motion of ssDNA in the presence of an applied electric field was primarily due to electrophoresis and was not influenced significantly by electro-osmotic flow. Our results also suggest that the use of asymmetric diffusion barriers or other tunable obstacles may assist DNA separation on supported lipid bilayers.