Chemical stabilization of cartilage matrix.
Chemical stabilization of cartilage matrix.
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软骨基质的化学稳定。
DOI：
10.1006/jsre.1994.1047
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Thonar,EJ
中科院分区：
文献类型：
--
作者：
Kahn,A;Pottenger,LA;Albertini,JG;Taitz,AD;Thonar,EJ
当修复过程跟不上分解代谢过程时，骨关节炎中关节软骨的严重破坏就会在临床上表现出来。蛋白多糖赋予组织进行可逆变形的能力，其损失先于关节软骨最表层基质的破坏，并可能对这种破坏产生重大影响。在这项研究中，我们检查了二硫双[琥珀酰亚胺基丙酸酯]（一种跨度为 1.2 nm 的双功能试剂，可在赖氨酸氨基酸处交联蛋白质）和高分子量聚 L-赖氨酸（平均 MW 360,000）减少牛鼻软骨和人髌软骨薄片（40 和 200 μm）中蛋白聚糖和胶原蛋白被动损失的能力在 4°C 的缓冲液中孵育 7 天。我们提供的证据表明，用这些药物中的任何一种治疗软骨薄片可有效减少软骨基质中蛋白聚糖和胶原蛋白的损失，并且我们定义了优化软骨基质稳定性的条件（治疗时间和所需浓度）。通过在修复过程中保护软骨细胞周围的环境，软骨基质的化学稳定可能成为骨关节炎治疗的重要方式。
Severe destruction of articular cartilage in osteoarthritis manifests clinically when repair processes cannot keep up with the catabolic processes. Loss of proteoglycans, which give the tissue its ability to undergo reversible deformation, precedes and probably contributes significantly to breakdown of the matrix in the most superficial layers of articular cartilage. In this study, we have examined the ability of dithiobis[succinimidyl proprionate], a bifunctional reagent with a 1.2-nm span that cross-links proteins at lysine amino acid, and poly-l-lysine of high molecular weight (average MW 360,000) to reduce passive loss of proteoglycans and collagen from thin slices (40 and 200 μm) of bovine nasal and human patellar cartilage incubated for 7 days in buffer at 4°C. We present evidence that treatment of thin slices of cartilage with either of these agents is effective in reducing the loss of proteoglycans and collagen from the cartilage matrix and we define conditions (length of treatment and concentrations required) under which the stabilization of the cartilage matrix is optimized. Chemical stabilization of cartilage matrix may become an important modality of treatment in osteoarthritis by protecting the environment around chondrocytes during the repair process.