Resveratrol promotes cellular glucose utilization in primary cultured cortical neurons via calcium-dependent signaling pathway
Resveratrol promotes cellular glucose utilization in primary cultured cortical neurons via calcium-dependent signaling pathway
复制标题
白藜芦醇通过钙依赖性信号通路促进原代培养的皮质神经元的细胞葡萄糖利用。
DOI：
10.1016/j.jnutbio.2012.02.015
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发表时间：
2013-04-01
影响因子：
5.6
通讯作者：
Chen, Jian-Guo
中科院分区：
文献类型：
--
作者：
Zhang, Jun-Qi;Wu, Peng-Fei;Chen, Jian-Guo
背景和目的：葡萄糖利用障碍是阿尔茨海默病患者神经元变性的重要原因。细胞葡萄糖利用可通过钙依赖性信号通路调节。白藜芦醇（RSV）是一种植物来源的多酚，具有多种有益作用，包括神经保护和代谢改善。本实验采用原代培养的大鼠皮层神经元，采用钙离子成像、2-NBDG测定和Western blotting等方法，研究RSV对神经元钙信号和葡萄糖利用的影响。其次，观察到RSV处理后神经元葡萄糖利用的钙依赖性增强。对神经元葡萄糖利用的影响在很大程度上依赖于RSV诱导的钙依赖性AMP激活的蛋白激酶activation.Conclusion：我们的研究结果表明，RSV激活钙依赖性信号通路可能传达神经元葡萄糖利用的改善。(C)2013 Elsevier Inc. All rights reserved.
Background and Purpose: Impairment of glucose utilization contributes to neuronal degeneration of Alzheimer's disease patients. Cellular glucose utilization can be regulated by calcium-dependent signaling pathways. Resveratrol (RSV) is a plant-derived polyphenol with multiple beneficial effects, including neuroprotection and metabolic improvement. Here, we investigated the effect of RSV on neuronal calcium signal and glucose utilization.Experimental Methods: Primary culture of cortical neurons, calcium imaging, 2-NBDG assay and western blotting were employed to investigate RSV-mediated effects on neuronal calcium signal and glucose utilization.Results: RSV elevated intracellular calcium in cortical neurons via modulation of secondary messenger system including nitrous oxide, cGMP and cAMP. Secondarily, a calcium-dependent enhancement of neuronal glucose utilization after RSV treatment was observed. The effects on neuronal glucose utilization are largely dependent on RSV-induced calcium-dependent AMP-activated protein kinase activation.Conclusion: Our findings show that activation of calcium-dependent signaling pathways by RSV may convey improvements of neuronal glucose utilization. (C) 2013 Elsevier Inc. All rights reserved.