Cloning, expression analysis, and chromosomal mapping of GTPBP2, a novel member of the G protein family
Cloning, expression analysis, and chromosomal mapping of GTPBP2, a novel member of the G protein family
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DOI：
10.1016/s0378-1119(00)00346-2
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发表时间：
2000-10-03
期刊：
影响因子：
3.5
通讯作者：
Sugano, S
中科院分区：
文献类型：
--
作者：
Watanabe, M;Yoshida, K;Sugano, S
我们已经鉴定了一个新的基因编码的蛋白带有GTP结合基序，GTP结合蛋白（G蛋白）的特征。推导的氨基酸序列与GTPBP 1及其小鼠直系同源物GP-1具有最高的整体同源性。因此，我们将该基因命名为GTPBP 2。该基因的小鼠直向同源物Gtpbp 2在整个蛋白质上与GTPBP 2显示出98%的同一性（HGMW批准的命名符号是GTPBP 2，小鼠直向同源物是Gtpbp 2）。系统发育分析表明，GTPBP 2和同源G蛋白（GTPBP 1，AGP-1，和CGP-1）不属于主要的G蛋白家族。它们在系统发育树中形成了一个独特的分支，表明它们构成了一个新的G蛋白家族。一个2.9 kb的mRNA主要在睾丸中检测到沿着各种其他器官。原位杂交分析表明，Gtpbp 2主要表达在睾丸的精母细胞和圆形精子细胞。这些新基因定位于人染色体6p21.1-2和小鼠染色体17 qC-D。(C)2000 Elsevier Science B. V.保留所有权利。
We have identified a novel gene encoding a protein bearing GTP-binding motifs, the characteristics of GTP-binding proteins (G proteins). The deduced amino acid sequence exhibited the highest overall homology with GTPBP1 and its mouse orthologue GP-I. Hence, we named the gene GTPBP2. The mouse orthologue of this gene, Gtpbp2, showed 98% identity with GTPBP2 over the entire protein (the HGMW-approved nomenclature symbol is GTPBP2 and mouse orthologue is Gtpbp2). A phylogenetic analysis showed GTPBP2 and homologous G proteins (GTPBP1, AGP-1, and CGP-1) did not belong to major G protein families. They formed a distinct branch in the phylogenetic tree, suggesting that they constitute a novel G protein family. A 2.9 kb mRNA was predominantly detected in the testis along with various other organs. In situ hybridization analysis revealed that Gtpbp2 was predominantly expressed in spermatocytes and round-spermatids in the testis. These novel genes were localized to human chromosome 6p21.1-2 and mouse chromosome 17qC-D. (C) 2000 Elsevier Science B.V. All rights reserved.