Uptake, metabolism, and cytotoxicity of doxorubicin in human Ewing's sarcoma and rhabdomyosarcoma cells
Uptake, metabolism, and cytotoxicity of doxorubicin in human Ewing's sarcoma and rhabdomyosarcoma cells
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DOI：
10.1007/bf00255912
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发表时间：
2004
影响因子：
3
通讯作者：
M. DeGregorio;G. Lui;B. Macher;J. Wilbur
中科院分区：
文献类型：
--
作者：
M. DeGregorio;G. Lui;B. Macher;J. Wilbur
Uptake, metabolism, and cytotoxicity of doxorubicin (DOX) in human Ewing's sarcoma (ES) and rhabdomyosarcoma (RS) cells were examined. Cellular uptake of DOX was determined by liquid scintillation spectrometry, and intracellular metabolism was examined by high performance liquid chromatography. The cytotoxicity of DOX was assessed by two different methods: an extracellular matrix detachment assay (ECM-D) and3H-thymidine incorporation. The uptake of DOX by ES cells was 1.5–3.0 times greater than RS cells, even though both cell types achieved intracellular steady-state concentrations between 6–8 h. No significant intracellular metabolism (< 5%) was seen after 8-h incubations with the drug. The cytotoxic effects of DOX in both cell lines were concentration-dependent, with the RS cells being more sensitive. Measurement of14C-DOX appears to be a reliable method for quantitating intracellular DOX. In addition, the ECM-D and3H-thymidine assays used for assessing cytotoxicity produced similar results, showing that the ECM-D can be a reliable and easily performed test of cell death.
