The effects of testosterone and estrogen on the pituitary growth hormone response to growth hormone-releasing factor.
The effects of testosterone and estrogen on the pituitary growth hormone response to growth hormone-releasing factor.
复制标题
睾酮和雌激素对垂体生长激素对生长激素释放因子反应的影响。
DOI：
10.1095/biolreprod32.2.369
复制
发表时间：
1985
影响因子：
3.6
通讯作者：
Ling,N
中科院分区：
文献类型：
--
作者：
Wehrenberg,WB;Baird,A;Ying,SY;Ling,N
在体内使用雄性和雌性大鼠和在体外使用垂体细胞单层培养系统评价睾酮和雌激素对垂体生长激素对下丘脑生长激素释放因子（GRF）的反应的影响。在体内，对500 ng/kg剂量的GRF的血浆生长激素（GH）浓度的增加在性腺切除的雄性和雌性大鼠中相似。用睾酮预处理完整和去性腺的雄性大鼠，可显著增强垂体GH对GRF的反应，而用17β-雌二醇预处理去性腺的雌性大鼠则不改变这种反应。GH对GRF的反应在青春期前（即，30日龄）雄性和雌性大鼠。然而，在青春期之后（即，到60日龄），雄性大鼠的反应明显大于雌性大鼠。垂体前叶细胞与睾酮或17β-雌二醇的体外预孵育均未引起GRF和GH之间剂量-反应曲线的任何偏移。这些结果表明，雄激素在体内调节垂体对GRF的反应中起积极作用。
The effects of testosterone and estrogen on the pituitary growth hormone response to hypothalamic growth hormone-releasing factor (GRF) were evaluated in vivo using male and female rats and in vitro using a pituitary cell monolayer culture system. In vivo the increase in plasma growth hormone (GH) concentration in response to a 500 ng/kg dose of GRF was similar in gonadectomized male and female rats. Pretreatment of intact and gonadectomized male rats with testosterone caused significant enhancement of the pituitary GH response to GRF, whereas pretreatment of gonadectomized female rats with 17β-estradiol did not alter the response. The GH response to GRF was not different between prepubertal (i.e., 30-day-old) male and female rats. However, following puberty (i.e., by 60 days of age), the response in male rats was significantly greater than that observed in female rats. The in vitro preincubation of anterior pituitary cells with either testosterone or 17β-estradiol did not cause any shift in the dose-response curve between GRF and GH. These results demonstrated that androgens play an active role in modulating the pituitary response to GRF in vivo.