Increasing tolerance to Cry1Ac cotton from cotton bollworm, Helicoverpa armigera, was confirmed in Bt cotton farming area of China
Increasing tolerance to Cry1Ac cotton from cotton bollworm, Helicoverpa armigera, was confirmed in Bt cotton farming area of China
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DOI：
10.1111/j.1365-2311.2007.00891.x
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发表时间：
2007-08-01
影响因子：
2.2
通讯作者：
Guo, Yu-Yuan
中科院分区：
文献类型：
--
作者：
Li, Guo-Ping;Wu, Kong-Ming;Guo, Yu-Yuan
2002-2005年，利用河北省安次县(玉米、大豆、花生、Bt棉复种系统)和山东省夏津县(Bt棉密集种植区)等雌系F-1和F-2后代的生物测定，研究了中国北部棉区棉铃虫对Cry1Ac毒素抗性基因频率的变化和耐性转变。对总体结果的保守分析表明，棉铃虫对Cry1Ac毒素的抗性频率略有增加，在F-1代棉铃虫幼虫的相对平均发育等级[Bt饲料品系的RADR生长速率与非Bt(NBT)饲料饲料的生长速率]逐年显著增加，表明田间种群对Cry1Ac的耐受性逐渐增强。F-1代品系的RADR与其F-2后代的RADR也呈显著正相关。定量遗传模拟分析表明，如果不采取有效的抗性管理措施，夏津棉区棉铃虫对Bt棉的抗性可在11~15年内进化到较高水平。
Changes in the frequency of Cry1Ac resistance genes and shifts in tolerance of cotton bollworm, Helicoverpa armigera, to the Cry1Ac toxin were assessed using bioassays of F-1 and F-2 offspring of isofemale lines from Anci County of Hebei Province (a multiple-crop system including corn, soybean, peanut, and Bt cotton) and Xiajin County of Shandong Province (an intensive Bt cotton planting area) in Northern China during 2002-2005.A conservative analysis of the overall results indicated that there was a small increase in the frequency of major, non-recessive resistance genes over time.The relative average development ratings [RADR - growth rate of a line on a Bt diet in proportion to the growth rate on a non-Bt (NBT) diet] of the bollworm larvae in F-1 tests increased significantly from year to year, indicating a gradual trend towards higher tolerance to Cry1Ac in the field populations.There were also significant positive correlations between RADR of the lines in the F-1 generation and the RADR of their F-2 offspring, indicating that the tolerance was genetically based.Quantitative genetic simulation analysis showed that resistance of H. armigera to Bt cotton in Xiajin could evolve to a high level in 11-15 years if no effective resistance management measures are carried out.